Risks associated with high-dose Lactobacillus rhamnosus in an Escherichia coli model of piglet diarrhoea: intestinal microbiota and immune imbalances.
Risks associated with high-dose Lactobacillus rhamnosus in an Escherichia coli model of piglet diarrhoea: intestinal microbiota and immune imbalances.
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DOI：
10.1371/journal.pone.0040666
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发表时间：
2012
期刊：
影响因子：
3.7
通讯作者：
Wang JF
中科院分区：
文献类型：
--
作者：
Li XQ;Zhu YH;Zhang HF;Yue Y;Cai ZX;Lu QP;Zhang L;Weng XG;Zhang FJ;Zhou D;Yang JC;Wang JF
益生菌可能是预防肠道感染的抗生素的有前途的替代品;然而，需要关于剂量效应的进一步信息。在这项研究中，断奶仔猪口服低或高剂量的鼠李糖乳杆菌ACTT 7469（1010 CFU/d或1012 CFU/d）1周前，F4（K88）阳性大肠杆菌的挑战。记录粪便和胃肠道菌群组成;实时荧光PCR检测肠道基因表达; ELISA和免疫组化分别检测血清肿瘤坏死因子-α（TNF-α）浓度和肠道Toll样受体4（TLR 4）。出乎意料的是，高剂量给药增加了F4 +ETEC攻毒前腹泻的发生率，尽管两种剂量均改善了F4+ ETEC诱导的腹泻，乳酸杆菌和双歧杆菌计数增加，粪便中大肠菌群脱落减少。有趣的是，L。鼠李糖乳杆菌和双歧杆菌的结肠内容物的计数减少，和高剂量的仔猪也有较低的乳酸杆菌和拟杆菌在回肠内容物的计数。观察到F4+ETEC诱导的血清TNF-α浓度升高，但L.鼠李糖在暴露于F4+ETEC的仔猪中，空肠TLR 4表达在mRNA和蛋白水平上增加，而空肠白细胞介素（IL）-8和回肠猪β-防御素2（pBD 2）mRNA表达增加;然而，这些增加通过给予L.鼠李糖值得注意的是，在F4+ETEC攻击后，低剂量仔猪空肠TLR 2、回肠TLR 9、Nod样受体NOD 1和TNF-α mRNA的表达上调，但在高剂量仔猪中未上调。提示低剂量L.鼠李糖可能比高剂量更有效地改善腹泻。高剂量L.鼠李糖预处理可能抵消预防作用，从而降低对潜在肠道病原体的预防益处。我们的数据表明，在临床实践中预防性使用益生菌的安全阈值。
Probiotic could be a promising alternative to antibiotics for the prevention of enteric infections; however, further information on the dose effects is required. In this study, weanling piglets were orally administered low- or high-dose Lactobacillus rhamnosus ACTT 7469 (1010 CFU/d or 1012 CFU/d) for 1 week before F4 (K88)-positive Escherichia coli challenge. The compositions of faecal and gastrointestinal microbiota were recorded; gene expression in the intestines was assessed by real-time PCR; serum tumour necrosis factor-α (TNF-α) concentrations and intestinal Toll-like receptor 4 (TLR4) were detected by ELISA and immunohistochemistry, respectively. Unexpectedly, high-dose administration increased the incidence of diarrhoea before F4+ETEC challenge, despite the fact that both doses ameliorated F4+ETEC-induced diarrhoea with increased Lactobacillus and Bifidobacterium counts accompanied by reduced coliform shedding in faeces. Interestingly, L. rhamnosus administration reduced Lactobacillus and Bifidobacterium counts in the colonic contents, and the high-dose piglets also had lower Lactobacillius and Bacteroides counts in the ileal contents. An increase in the concentration of serum TNF-α induced by F4+ETEC was observed, but the increase was delayed by L. rhamnosus. In piglets exposed to F4+ETEC, jejunal TLR4 expression increased at the mRNA and protein levels, while jejunal interleukin (IL)-8 and ileal porcine β-defensins 2 (pBD2) mRNA expression increased; however, these increases were attenuated by administration of L. rhamnosus. Notably, expression of jejunal TLR2, ileal TLR9, Nod-like receptor NOD1 and TNF-α mRNA was upregulated in the low-dose piglets after F4+ETEC challenge, but not in the high-dose piglets. These findings indicate that pretreatment with a low dose of L. rhamnosus might be more effective than a high dose at ameliorating diarrhoea. There is a risk that high-dose L. rhamnosus pretreatment may negate the preventative effects, thus decreasing the prophylactic benefits against potential enteric pathogens. Our data suggest a safe threshold for preventative use of probiotics in clinical practice.
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影响因子：
4.4
作者：
Daudelin JF;Lessard M;Beaudoin F;Nadeau E;Bissonnette N;Boutin Y;Brousseau JP;Lauzon K;Fairbrother JM
通讯作者：
Fairbrother JM
影响因子：
2.2
作者：
Liu, Fangning;Li, Guohua;Yuan, Lijuan
通讯作者：
Yuan, Lijuan
影响因子：
3.3
作者：
Frydendahl, K
通讯作者：
Frydendahl, K
影响因子：
64.8
作者：
Bouskra, Djahida;Brezillon, Christophe;Eberl, Gerard
通讯作者：
Eberl, Gerard
影响因子：
2.6
作者：
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通讯作者：
Salminen, Seppo