Administration of probiotics influences F4 (K88)-positive enterotoxigenic Escherichia coli attachment and intestinal cytokine expression in weaned pigs.
Administration of probiotics influences F4 (K88)-positive enterotoxigenic Escherichia coli attachment and intestinal cytokine expression in weaned pigs.
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DOI：
10.1186/1297-9716-42-69
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发表时间：
2011-05-23
影响因子：
4.4
通讯作者：
Fairbrother JM
中科院分区：
文献类型：
--
作者：
Daudelin JF;Lessard M;Beaudoin F;Nadeau E;Bissonnette N;Boutin Y;Brousseau JP;Lauzon K;Fairbrother JM
本研究评价了益生菌乳酸片球菌和布拉氏酿酒酵母对携带F4（K88）菌毛的O 149产肠球菌性大肠杆菌（ETEC F4）肠道定殖以及对断奶仔猪回肠细胞因子表达的影响。出生时，将不同窝的猪随机分配至以下处理之一：1）不含抗生素或益生菌的对照组（CTRL）; 2）在断奶饲料中添加金霉素和泰妙菌素的参考组（ATB）; 3）乳酸丙酸杆菌; 4）沙门氏菌。酿酒酵母布拉氏菌（P. acidilacidum）+ S.布拉氏酿酒酵母在哺乳期和断奶后（第21天），每天给予益生菌（每头猪1 × 109 CFU）。在28日龄时，所有猪均经口接种ETEC F4菌株，24小时后进行尸检。收集肠段以评估小肠中的细菌定植和回肠细胞因子表达。在用乳酸丙酸杆菌或沙门氏菌处理的猪中，ETEC F4与肠粘膜的附着显著减少。与ATB组相比，酿酒酵母布拉氏菌（P = 0.01和P = 0.03）。此外，在ETEC F4攻毒猪中，单用酸乳假单胞菌或与沙门氏菌联合给药的促炎细胞因子（如IL-6）上调。与对照组相比，总之，施用P. acidilaclavidine或S.酿酒酵母布拉氏菌可有效减少ETEC F4与回肠粘膜的粘附，而在ETEC F4攻毒猪中，需要存在乳酸假单胞菌才能调节肠道炎性细胞因子的表达。
This study evaluated the effect of the probiotics Pediococcus acidilactici and Saccharomyces cerevisiae boulardii on the intestinal colonization of O149 enterotoxigenic Escherichia coli harbouring the F4 (K88) fimbriae (ETEC F4) and on the expression of ileal cytokines in weaned pigs. At birth, different litters of pigs were randomly assigned to one of the following treatments: 1) control without antibiotics or probiotics (CTRL); 2) reference group in which chlortetracycline and tiamulin were added to weanling feed (ATB); 3) P. acidilactici; 4) S. cerevisiae boulardii; or 5) P. acidilactici + S. cerevisiae boulardii. Probiotics were administered daily (1 × 109 CFU per pig) during the lactation period and after weaning (day 21). At 28 days of age, all pigs were orally challenged with an ETEC F4 strain, and a necropsy was performed 24 h later. Intestinal segments were collected to evaluate bacterial colonization in the small intestine and ileal cytokine expressions. Attachment of ETEC F4 to the intestinal mucosa was significantly reduced in pigs treated with P. acidilactici or S. cerevisiae boulardii in comparison with the ATB group (P = 0.01 and P = 0.03, respectively). In addition, proinflammatory cytokines, such as IL-6, were upregulated in ETEC F4 challenged pigs treated with P. acidilactici alone or in combination with S. cerevisiae boulardii compared with the CTRL group. In conclusion, the administration of P. acidilactici or S. cerevisiae boulardii was effective in reducing ETEC F4 attachment to the ileal mucosa, whereas the presence of P. acidilactici was required to modulate the expression of intestinal inflammatory cytokines in pigs challenged with ETEC F4.
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影响因子：
3.1
作者：
Miettinen, M;Matikainen, S;Julkunen, I
通讯作者：
Julkunen, I
DOI：
10.1016/j.bbrc.2006.02.080
发表时间：
2006-04-28
影响因子：
3.1
作者：
Sougioultzis, S;Simeonidis, S;Kelly, CP
通讯作者：
Kelly, CP
影响因子：
4.6
作者：
Schlee, M.;Harder, J.;Fellermann, K.
通讯作者：
Fellermann, K.
影响因子：
24.5
作者：
Haller, D;Bode, C;Blum, S
通讯作者：
Blum, S
影响因子：
3.3
作者：
Lessard, M.;Dupuis, M.;Fairbrother, J. M.
通讯作者：
Fairbrother, J. M.