Kinetics of high-affinity Ca2+ sequestration in permeabilized rat pancreatic acini.
Kinetics of high-affinity Ca2+ sequestration in permeabilized rat pancreatic acini.
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透化大鼠胰腺腺泡中高亲和力 Ca2 隔离的动力学。
DOI：
10.1152/ajpcell.1988.254.5.c621
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发表时间：
1988
期刊：
影响因子：
--
通讯作者：
Hurley,TW
中科院分区：
文献类型：
--
作者：
Hurley,TW
能量依赖性的亚细胞Ca 2+螯合研究钌红的存在下，使用大鼠胰腺腺泡，这已被透化暴露于介质名义上免费的Ca 2+。Ca 2+摄取的初始速率（约2，800 pmol.min-1.mg腺泡蛋白-1）迅速减慢，在37 ℃孵育5-10分钟后，达到约3，000 pmol/mg的平均稳态Ca 2+含量。亚微摩尔Ca 2+浓度（K0.5 = 156 nM）刺激Ca 2+吸收;所需的Mg 2 +-ATP（K0.5 = 0.78 mM）在pH 7.0时最大，并被Ca 2+离子载体A23187废除。其他核苷酸磷酸盐以及对硝基苯基磷酸盐是相对较差的底物，支持在ATP存在下测量的初始速率的6-14%的Ca 2+摄取。这些结果表明，胰腺腺泡透性无洗涤剂具有非线粒体Ca 2+转运系统不位于质膜，但预期的一个主要的调节器的性能的腺泡胞浆Ca 2+浓度。
Energy-dependent subcellular Ca2+ sequestration was studied in the presence of ruthenium red using rat pancreatic acini, which had been permeabilized by exposure to medium nominally free of Ca2+. The initial rate of Ca2+ uptake (approximately 2,800 pmol.min-1.mg acinar protein-1) quickly slowed, and a mean steady-state Ca2+ content of approximately 3,000 pmol/mg was reached after 5-10 min of incubation at 37 degrees C. Ca2+ uptake was stimulated by submicromolar Ca2+ concentrations (K0.5 = 156 nM); required Mg2+-ATP (K0.5 = 0.78 mM) was greatest at a pH of 7.0 and was abolished by the Ca2+ ionophore A23187. Other nucleotide phosphates as well as p-nitrophenylphosphate were relatively poor substrates, supporting Ca2+ uptake at initial rates that were 6-14% of those measured in the presence of ATP. These results show that pancreatic acini permeabilized without detergents possess a nonmitochondrial Ca2+ transporting system not located in the plasma membrane but with the properties expected of a major regulator of acinar cytosolic Ca2+ concentration.
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豚鼠腮腺分离微粒体中 ATP 依赖性钙封存和钙/ATP 化学计量
影响因子：
3.5
作者：
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通讯作者：
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DOI：
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发表时间：
1984
期刊：
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影响因子：
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作者：
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通讯作者：
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影响因子：
56.9
作者：
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通讯作者：
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影响因子：
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作者：
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