Nfil3/E4bp4 is required for the development and maturation of NK cells in vivo.
Nfil3/E4bp4 is required for the development and maturation of NK cells in vivo.
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NFIL3/E4BP4是在体内开发和成熟所必需的。
DOI：
10.1084/jem.20092176
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发表时间：
2009-12-21
期刊：
影响因子：
--
通讯作者：
Mak TW
中科院分区：
文献类型：
--
作者：
Kamizono S;Duncan GS;Seidel MG;Morimoto A;Hamada K;Grosveld G;Akashi K;Lind EF;Haight JP;Ohashi PS;Look AT;Mak TW
核因子白细胞介素-3（Nfil 3;也称为E4结合蛋白4）是一种碱性区域亮氨酸拉链转录因子，在生长因子撤除条件下具有体外抗凋亡活性。为了研究Nfil 3在体内的作用，我们产生了基因靶向的Nfil 3缺陷（Nfil 3 −/−）小鼠。Nfil 3 −/−小鼠以正常孟德尔频率出生，并且大体上正常且可生育。尽管Nfil 3 −/−小鼠的T细胞、B细胞和自然杀伤（NK）T细胞数量正常，但NK细胞发育的特定中断导致外周成熟NK细胞数量严重减少。这种缺陷本质上是NK细胞固有的，导致在体内不能排斥MHC I类缺陷细胞，并在体外降低干扰素γ的产生和细胞溶解活性。我们的结果证实了Nfil 3对NK谱系细胞发育的特异性和基本要求。
Nuclear factor interleukin-3 (Nfil3; also known as E4-binding protein 4) is a basic region leucine zipper transcription factor that has antiapoptotic activity in vitro under conditions of growth factor withdrawal. To study the role of Nfil3 in vivo, we generated gene-targeted Nfil3-deficient (Nfil3−/−) mice. Nfil3−/− mice were born at normal Mendelian frequency and were grossly normal and fertile. Although numbers of T cells, B cells, and natural killer (NK) T cells were normal in Nfil3−/− mice, a specific disruption in NK cell development resulted in severely reduced numbers of mature NK cells in the periphery. This defect was NK cell intrinsic in nature, leading to a failure to reject MHC class I–deficient cells in vivo and reductions in both interferon γ production and cytolytic activity in vitro. Our results confirm the specific and essential requirement of Nfil3 for the development of cells of the NK lineage.
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影响因子：
56.9
作者：
INABA, T;ROBERTS, WM;LOOK, AT
通讯作者：
LOOK, AT
DOI：
10.1073/pnas.94.6.2609
发表时间：
1997-03-18
影响因子：
11.1
作者：
Ikushima, S;Inukai, T;Look, AT
通讯作者：
Look, AT
影响因子：
20.3
作者：
Pellegrini, M;Bath, S;Strasser, A
通讯作者：
Strasser, A
影响因子：
5.4
作者：
Ishida, H;Ueda, K;Hayashi, N
通讯作者：
Hayashi, N
影响因子：
32.4
作者：
Park, SY;Saijo, K;Saito, T
通讯作者：
Saito, T