Two distinct pools of large-conductance calcium-activated potassium channels in the somatic plasma membrane of central principal neurons.
Two distinct pools of large-conductance calcium-activated potassium channels in the somatic plasma membrane of central principal neurons.
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DOI：
10.1016/j.neuroscience.2010.05.070
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发表时间：
2010-09-01
期刊：
影响因子：
3.3
通讯作者：
Storm, J. F.
中科院分区：
文献类型：
--
作者：
Kaufmann, W. A.;Kasugai, Y.;Ferraguti, F.;Storm, J. F.
关键词：
虽然神经细胞膜通常被认为是均匀的电性能方面，有越来越多的证据划分成子域的整体细胞功能的异质性的影响。这些微结构域的特征在于决定其功能特性的特定蛋白质组。最近，集群的大电导钙激活钾（BKCa）通道显示在网站的亚表面膜池在小脑浦肯野细胞（PC），在那里他们可能参与建设一个亚细胞信号单位，'PLASMER小体'。应用SDS-消化的冷冻断裂复制标记（SDS-FRL）和植入后免疫金电子显微镜，我们现在已经研究了新皮层第5层锥体神经元、中央和基底外侧杏仁核的主要神经元、海马锥体神经元和齿状回（DG）颗粒细胞，以确定是否有一个共同的组织原则，在中央主神经元的BKCa通道的分布。在分析的所有细胞类型中，发现体细胞BKCa通道在质膜中非均匀分布，形成两个通道池，其中一个池由聚集的通道组成，另一个池由突触外膜中的分散通道组成。SDS-FRL定量分析表明，在主细胞胞体中，约三分之二的BKCa通道属于散在池，约三分之一属于聚集池。两个池中通道的总体密度在分析的不同细胞类型中不同，尽管与小脑PC相比显著较低。植入后的免疫金标记显示关联的集群通道与亚表面膜cispellant和确认突触外本地化的分散的渠道。这项研究表明，一个共同的组织原则，体细胞BKCa通道在中央主神经元的通道形成的集群和分散的池，这种通道类型的细胞类型的特定密度。
Although nerve cell membranes are often assumed to be uniform with respect to electrical properties, there is increasing evidence for compartmentalization into subdomains with heterogeneous impacts on the overall cell function. Such microdomains are characterized by specific sets of proteins determining their functional properties. Recently, clustering of large-conductance calcium-activated potassium (BKCa) channels was shown at sites of subsurface membrane cisterns in cerebellar Purkinje cells (PC), where they likely participate in building a subcellular signaling unit, the 'PLasmERosome'. By applying SDS-digested freeze-fracture replica labeling (SDS-FRL) and postembedding immunogold electron microscopy, we have now studied the spatial organization of somatic BKCa channels in neocortical layer 5 pyramidal neurons, principal neurons of the central and basolateral amygdaloid nuclei, hippocampal pyramidal neurons and dentate gyrus (DG) granule cells to establish whether there is a common organizational principle in the distribution of BKCa channels in central principal neurons. In all cell types analyzed, somatic BKCa channels were found to be non-homogenously distributed in the plasma membrane, forming two pools of channels with one pool consisting of clustered channels and the other of scattered channels in the extrasynaptic membrane. Quantitative analysis by means of SDS-FRL revealed that about two-thirds of BKCa channels belong to the scattered pool and about one-third to the clustered pool in principal cell somata. Overall densities of channels in both pools differed in the different cell types analyzed, although being considerably lower compared to cerebellar PC. Postembedding immunogold labeling revealed association of clustered channels with subsurface membrane cisterns and confirmed extrasynaptic localization of scattered channels. This study indicates a common organizational principle for somatic BKCa channels in central principal neurons with the formation of a clustered and a scattered pool of channels, and a cell-type specific density of this channel type.
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DOI：
10.1085/jgp.109.5.647
发表时间：
1997-05
期刊：
The Journal of general physiology
影响因子：
--
作者：
Cui J;Cox DH;Aldrich RW
通讯作者：
Aldrich RW
影响因子：
4
作者：
Berridge, Michael J.
通讯作者：
Berridge, Michael J.
影响因子：
5.3
作者：
Hu, H;Shao, LR;Storm, JF
通讯作者：
Storm, JF
影响因子：
4.8
作者：
KNAUS, HG;EBERHART, A;GARCIA, ML
通讯作者：
GARCIA, ML
影响因子：
5.5
作者：
Edgerton, JR;Reinhart, PH
通讯作者：
Reinhart, PH