Exploration of microRNAs and their targets engaging in the resistance interaction between wheat and stripe rust.
Exploration of microRNAs and their targets engaging in the resistance interaction between wheat and stripe rust.
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小麦与条锈病抗性相互作用的 microRNA 及其靶标的探索
DOI：
10.3389/fpls.2015.00469
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发表时间：
2015
影响因子：
5.6
通讯作者：
Kang Z
中科院分区：
文献类型：
--
作者：
Feng H;Wang B;Zhang Q;Fu Y;Huang L;Wang X;Kang Z
小麦条锈病是由小麦条锈菌（Pucciniastriiformis f.）小麦赤霉病（Pst）是世界性的小麦病害之一。miRNAs是植物重要的调控因子，在植物器官发育、植物阶段性变化和防御反应中起着重要的作用。本研究以小麦品种兴资9104（XZ）为材料，构建了两个分别在Pst无毒小种CYR 32和无菌水胁迫下表达的miRNA文库。共获得596个候选miRNA。使用基于微阵列的分析在用Pst攻击的成年植物中筛选420个小麦特异性候选miRNAs。我们分析了候选miRNAs的丰度，并通过定量真实的时间PCR（qRT-PCR）确定候选miRNAs子集的水平。qRT-PCR结果表明，部分miRNAs参与了小麦与Pst的不亲和互作。此外，我们还发现了一些在不同叶片中差异表达的miRNAs。利用degradome测序技术对小麦miRNAs的靶基因进行了鉴定。大多数注释的靶基因与信号转导、能量代谢等功能有关。通过qRT-PCR方法分析了XZ与CYR 32之间的相互作用，发现RabGAP/TBC结构域蛋白、锌指蛋白和富含半胱氨酸的受体样蛋白激酶41可能在XZ与CYR 32之间的不相容相互作用中起重要作用。有趣的是，miRNAs和靶基因似乎形成了一个复杂的调控网络，调节小麦-Pst相互作用。这些结果为进一步研究miRNAs在小麦-Pst互作中的重要调控作用奠定了基础。
Wheat stripe rust, caused by Puccinia striiformis f. sp. tritici (Pst), is one of the most destructive diseases of wheat worldwide. miRNAs are important regulators, they play very central roles in plant organ development, vegetable phase change and defense responses. In this study, two miRNA libraries from wheat cultivar Xingzi 9104 (XZ) challenged with the avirulent Pst race CYR32 and sterile water were constructed, respectively. A total of 596 miRNA candidates were obtained. 420 wheat-specific candidate miRNAs were screened in adult plants challenged with Pst using microarray-based analyses. We analyzed the abundance of candidate miRNAs, and the levels of a subset of candidate miRNAs were determined by quantitative real time PCR (qRT-PCR). The qRT-PCR results indicated that some miRNAs were involved in the incompatible interaction between wheat and Pst. In addition, we identified some miRNAs differentially expressed in different leaves. Additionally, the target genes of wheat miRNAs were confirmed by using degradome sequencing technology. Most of the annotated target genes are related to signal transduction, energy metabolism, and other functions. We selected some target genes for relative expression analysis using qRT-PCR, and found that RabGAP/TBC domain-containing protein, zinc finger protein and Cysteine-rich receptor-like protein kinase 41 may play important role in the incompatible interaction between XZ and CYR32. Intriguingly, miRNAs and target gene seem to form a complicated regulation network that regulates the wheat-Pst interaction. Our data provide the foundation for evaluating the important regulatory roles of miRNAs in the wheat-Pst interaction.
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影响因子：
3.7
作者：
Fahlgren N;Howell MD;Kasschau KD;Chapman EJ;Sullivan CM;Cumbie JS;Givan SA;Law TF;Grant SR;Dangl JL;Carrington JC
通讯作者：
Carrington JC
影响因子：
9.2
作者：
Addo-Quaye, Charles;Eshoo, Tifani W.;Axtell, Michael J.
通讯作者：
Axtell, Michael J.
小麦钙结合 EF-hand 蛋白基因 TaCab1 的克隆和表征及其响应条锈菌 f 的表达。
影响因子：
2.8
作者：
Feng, Hao;Wang, Xiaomin;Kang, Zhensheng
通讯作者：
Kang, Zhensheng
影响因子：
2.8
作者：
Gupta, Om Prakash;Permar, Vipin;Praveen, Shelly
通讯作者：
Praveen, Shelly
影响因子：
11.6
作者：
Aukerman, MJ;Sakai, H
通讯作者：
Sakai, H