Mass spectrometry-based proteomic analysis of potential infectious bursal disease virus VP3-interacting proteins in chicken embryo fibroblasts cells
Mass spectrometry-based proteomic analysis of potential infectious bursal disease virus VP3-interacting proteins in chicken embryo fibroblasts cells
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基于质谱的蛋白质组学分析鸡胚成纤维细胞中潜在传染性法氏囊病病毒 VP3 相互作用蛋白
DOI：
10.1007/s11262-021-01828-x
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发表时间：
2021-02
期刊：
影响因子：
1.6
通讯作者：
Qian Jing
中科院分区：
文献类型：
--
作者：
Ma Sun-Ting;Wang Yong-Shan;Wang Xiao-Li;Xia Xing-Xia;Bi Zhen-Wei;Wang Jing-Yu;Zhu Yu-Mei;Ouyang Wei;Qian Jing
传染性法氏囊病病毒（IBDV）结构蛋白VP 3在病毒装配、复制、免疫逃逸和抗凋亡等过程中起着重要作用。VP 3和宿主蛋白因子之间的相互作用可以影响病毒复制周期的各个阶段。本研究利用液相色谱-串联质谱（LC-MS/MS）技术，从137个与VP 3蛋白相互作用的宿主蛋白中筛选出了137个蛋白质。通过生物信息学分析，获得了这些蛋白的功能和相关性。大多数VP 3相互作用蛋白与结合、催化活性和结构分子活性相关，并在细胞部分和细胞中发挥功能。VP 3相互作用蛋白的生物学功能主要与“细胞骨架”、“翻译”和“信号转导机制”有关，涉及核糖体、“紧密连接”、肌动蛋白细胞骨架调节等途径。在宿主细胞中的6个潜在的VP 3相互作用蛋白被敲除，并且发现波形蛋白、肌球蛋白9和膜联蛋白A2与IBDV复制相关。本研究将有助于探索IBDV感染细胞的调控途径和细胞机制，并为深入研究VP 3的生物学功能和IBDV的复制或致病机制提供线索。
The structural protein VP3 of infectious bursal disease virus (IBDV) plays a critical role in viral assembly, replication, immune escape, and anti-apoptosis. Interaction between VP3 and host protein factors can affect stages in the viral replication cycle. In this study, 137 host proteins interacting with VP3 protein were screened through liquid chromatography–tandem mass spectrometry (LC–MS/MS)-based proteomics approach. The functions and relevance of the proteins were obtained through bioinformatics analysis. Most VP3-interacting proteins were linked to binding, catalytic activity, and structural molecular activity, and performed functions in cell parts and cells. Biological functions of VP3-interacting proteins were mainly relevant to "Cytoskeleton", "Translation", and "Signal transduction mechanisms", involving ribosomes, "Tight junction", regulation of actin cytoskeleton, and other pathways. Six potential VP3-interacting proteins in host cells were knocked down, and vimentin, myosin-9, and annexin A2 were found to be related to IBDV replication. This study would help explore regulatory pathways and cellular mechanisms in IBDV-infected cells, and also provided clues for the in-depth study of VP3 biological functions and IBDV replication or pathogenesis.
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影响因子：
14.9
作者：
Kanehisa M;Araki M;Goto S;Hattori M;Hirakawa M;Itoh M;Katayama T;Kawashima S;Okuda S;Tokimatsu T;Yamanishi Y
通讯作者：
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3.7
作者：
Fujita T;Kitaura F;Fujii H
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Fujii H
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Maraver, A;Oña, A;Rodriguez, JF
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Rodriguez, JF
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3.4
作者：
Du, Cheng;Liu, Hai-Fang;Zhou, Jian-Hua
通讯作者：
Zhou, Jian-Hua
影响因子：
5.4
作者：
Ye, Chengjin;Jia, Lu;Zhou, Jiyong
通讯作者：
Zhou, Jiyong