Decreased fragile histidine triad gene protein expression is associated with worse prognosis in oral squamous carcinoma.
Decreased fragile histidine triad gene protein expression is associated with worse prognosis in oral squamous carcinoma.
复制标题
脆性组氨酸三联体基因蛋白表达的降低与口腔鳞癌的预后较差相关。
DOI：
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发表时间：
2009
影响因子：
4.6
通讯作者：
R. Robinson
中科院分区：
文献类型：
--
作者：
L. Guerin;H. Hoffman;M. Zimmerman;R. Robinson
上下文
脆性组氨酸三联体（FHIT）基因被认为是一种肿瘤抑制基因，在各种肿瘤中的表达异常已被报道。
目的
目的探讨口腔鳞状细胞癌患者FHIT蛋白表达异常或FHIT基因杂合性缺失是否与患者生存率及其他临床指标相关。
设计
对53例口腔鳞状细胞癌初次手术治疗的患者进行了至少5年或直至死亡的随访。所有患者的FHIT蛋白表达通过免疫组化进行了研究，并使用福尔马林固定的石蜡包埋组织研究了20例患者的等位基因杂合性丢失和微卫星不稳定性。
结果
在5年随访中，肿瘤FHIT表达降低的患者中有61%死于疾病，肿瘤FHIT表达保留的患者中有37%死于疾病。对数秩分析显示，保留FHIT表达的患者具有较长的总生存期（P = .03）和无病生存期（P = .01）。FHIT的表达与淋巴结状态及临床分期无关。杂合性丢失见于10/20（50%）肿瘤，低水平的微卫星不稳定性见于4/20（20%）肿瘤，高水平的微卫星不稳定性见于1/20（5%）肿瘤。
结论
当口腔鳞状细胞癌患者中FHIT基因表达降低时，其与较差的生存结局相关。基因的杂合性丢失是常见的，但没有发现与蛋白质表达的相关性。杂合性丢失或微卫星不稳定性被发现与生存相关。由于涉及FHIT基因杂合性缺失的基因组改变与这些肿瘤中的蛋白质表达无关，因此FHIT表达的存在或不存在可能受其他因素控制。
CONTEXT
Fragile histidine triad (FHIT) gene is thought to be a tumor suppressor; abnormalities in expression have been reported in a variety of neoplasms.
OBJECTIVE
To determine whether abnormalities of FHIT protein expression or loss of heterozygosity in the FHIT gene were correlated with survival or other clinical parameters in patients with oral cavity squamous cell carcinoma.
DESIGN
Fifty-three patients with initial surgical treatment of oral cavity squamous cell carcinoma were followed a minimum of 5 years or until death. The FHIT protein expression was studied by immunohistochemistry in all patients, and a subset of 20 patients was studied for allelic loss of heterozygosity and microsatellite instability using formalin-fixed, paraffin-embedded tissue.
RESULTS
Sixty-one percent of patients whose tumors had reduced FHIT expression were dead of disease, and 37% of patients whose tumors exhibited preserved FHIT expression were dead of disease at 5-year follow-up. Log-rank analysis showed that patients retaining FHIT expression had a longer overall survival (P = .03) and disease-free survival (P = .01). The FHIT expression was not correlated with node status or clinical stage. Loss of heterozygosity was seen in 10 (50%) of 20 tumors, low levels of microsatellite instability in 4 (20%) of 20 tumors, and high levels of microsatellite instability in 1 (5%) of 20 tumors tested.
CONCLUSIONS
The FHIT gene was associated with a worse survival outcome when its expression was reduced in patients with oral cavity squamous cell carcinoma. Loss of heterozygosity in the gene was common, but no correlation with protein expression was found. Neither loss of heterozygosity nor microsatellite instability was found to correlate with survival. Because genomic alterations involving loss of heterozygosity of the FHIT gene were not associated with protein expression in these tumors, the presence or absence of FHIT expression may be controlled by other factors.
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影响因子：
11.2
作者：
D. Greenspan;D. Connolly;R. Wu;R. Y. Lei;Joshua T C Vogeistein;Young-Tak Kim;J. Mok;Nubia Mufloz;F. Bosch;K. Shah;Kathleen R. Cho;Spain
通讯作者：
D. Greenspan;D. Connolly;R. Wu;R. Y. Lei;Joshua T C Vogeistein;Young-Tak Kim;J. Mok;Nubia Mufloz;F. Bosch;K. Shah;Kathleen R. Cho;Spain
影响因子：
11.2
作者：
Janet I. Lee;Jean-Charles Soria;Khaled A. Hassan;Diane Liu;Ximing Tang;Adel K. El-Naggar;W. K. Hong-W.
通讯作者：
Janet I. Lee;Jean-Charles Soria;Khaled A. Hassan;Diane Liu;Ximing Tang;Adel K. El-Naggar;W. K. Hong-W.
原发性肺肿瘤和具有 FHIT 基因异常的细胞系中缺乏 Fhit 蛋白。
影响因子：
11.2
作者：
Sozzi,G;Tornielli,S;Tagliabue,E;Sard,L;Pezzella,F;Pastorino,U;Minoletti,F;Pilotti,S;Ratcliffe,C;Veronese,ML;Goldstraw,P;Huebner,K;Croce,CM;Pierotti,MA
通讯作者：
Pierotti,MA
DOI：
10.1073/pnas.94.25.13771
发表时间：
1997-12-09
影响因子：
11.1
作者：
Siprashvili, Z;Sozzi, G;Huebner, K
通讯作者：
Huebner, K