Production of itaconic acid from alkali pretreated lignin by dynamic two stage bioconversion.
Production of itaconic acid from alkali pretreated lignin by dynamic two stage bioconversion.
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DOI：
10.1038/s41467-021-22556-8
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发表时间：
2021-04-15
影响因子：
16.6
通讯作者：
Guss AM
中科院分区：
文献类型：
--
作者：
Elmore JR;Dexter GN;Salvachúa D;Martinez-Baird J;Hatmaker EA;Huenemann JD;Klingeman DM;Peabody GL 5th;Peterson DJ;Singer C;Beckham GT;Guss AM
扩大可以从木质素中提取的产品组合将是实现可行的生物经济的关键。在这里，我们设计了恶臭假单胞菌，用于从木质素衍生的模型芳香化合物和芳香族化合物中高产地生产三元酸循环衍生的积木化学物质衣康酸。我们开发了一种检测氮饥饿的生物传感器，用于动态两阶段生物生产，其中衣康酸是在非生长相关的生产阶段生产的。通过两条不同的衣康酸产生途径、三氯乙酸循环基因表达调控、竞争途径删除和动态调控，对香豆酸的产率和滴度分别达到56%和1.3molg/L，碱处理木素单体芳香族化合物的滴度达到1.4molg/ / 。这项工作说明了一个原理证明，在碳进入中央代谢后，使用动态代谢控制来改变碳的路线，通过减轻结构性表达有毒异源途径的负担，能够高产率地从木质素中生产有价值的化学物质。木质素转化为更高价值的产品对木质纤维生物精炼厂的经济生存至关重要。在这里，作者展示了利用信号放大的氮限氮生物传感器，通过动态两步发酵将碱处理过的木质素生物转化为衣康酸。
Expanding the portfolio of products that can be made from lignin will be critical to enabling a viable bio-based economy. Here, we engineer Pseudomonas putida for high-yield production of the tricarboxylic acid cycle-derived building block chemical, itaconic acid, from model aromatic compounds and aromatics derived from lignin. We develop a nitrogen starvation-detecting biosensor for dynamic two-stage bioproduction in which itaconic acid is produced during a non-growth associated production phase. Through the use of two distinct itaconic acid production pathways, the tuning of TCA cycle gene expression, deletion of competing pathways, and dynamic regulation, we achieve an overall maximum yield of 56% (mol/mol) and titer of 1.3 g/L from p-coumarate, and 1.4 g/L titer from monomeric aromatic compounds produced from alkali-treated lignin. This work illustrates a proof-of-principle that using dynamic metabolic control to reroute carbon after it enters central metabolism enables production of valuable chemicals from lignin at high yields by relieving the burden of constitutively expressing toxic heterologous pathways. Lignin conversion to higher value products is essential to the economic viability of lignocellulosic biorefineries. Here, the authors demonstrate the bioconversion of alkali pretreated lignin to itaconic acid by dynamic two stage fermentation using a signal-amplified nitrogen-limitation biosensor.
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影响因子：
3.8
作者：
Harder BJ;Bettenbrock K;Klamt S
通讯作者：
Klamt S
影响因子：
3.8
作者：
Jeon, Ho-Geun;Cheong, Dae-Eun;Choi, Jong Hyun
通讯作者：
Choi, Jong Hyun
影响因子：
3.2
作者：
Hervas, Ana B.;Canosa, Ines;Santero, Eduardo
通讯作者：
Santero, Eduardo
影响因子：
5.7
作者：
Geiser E;Przybilla SK;Friedrich A;Buckel W;Wierckx N;Blank LM;Bölker M
通讯作者：
Bölker M
影响因子：
5.7
作者：
Fernández M;Duque E;Pizarro-Tobías P;Van Dillewijn P;Wittich RM;Ramos JL
通讯作者：
Ramos JL