Microbial responses to xenobiotic compounds. Identification of genes that allow Pseudomonas putida KT2440 to cope with 2,4,6-trinitrotoluene.
Microbial responses to xenobiotic compounds. Identification of genes that allow Pseudomonas putida KT2440 to cope with 2,4,6-trinitrotoluene.
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DOI：
10.1111/j.1751-7915.2009.00085.x
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发表时间：
2009-03
影响因子：
5.7
通讯作者：
Ramos JL
中科院分区：
文献类型：
--
作者：
Fernández M;Duque E;Pizarro-Tobías P;Van Dillewijn P;Wittich RM;Ramos JL
恶臭假单胞菌 KT2440 在存在 2,4,6-三硝基甲苯 (TNT) 的情况下在含有葡萄糖的 M9 基本培养基中生长，其生长速度与不存在 TNT 时相似，尽管使用 DNA 微阵列的全局转录分析表明 TNT 会产生一些压力。对 TNT 应激的反应在转录水平上受到调节，观察到 65 个基因表达水平的显着变化。在这些基因中，39 个出现上调，26 个出现下调。上调基因的特性表明恶臭假单胞菌使用两种策略来克服 TNT 毒性：（i）诱导编码硝基还原酶和解毒相关酶（pnrA、xenD、acpD）的基因和（ii）诱导多药外排泵基因（mexEF/oprN）以降低细胞内 TNT 浓度。对 13 个上调基因和 7 个下调基因的突变体进行 TNT 毒性分析，揭示了 MexE/MexF/OprN 泵和假定的异喹啉 1-氧化还原酶在 TNT 耐受性中的作用。 ORF PP1232的转录水平不会因TNT而改变，但在硝基芳香族化合物存在的情况下会影响生长，并且在对4000个随机产生的突变体的筛选中发现了这一点。
Pseudomonas putida KT2440 grows in M9 minimal medium with glucose in the presence of 2,4,6‐trinitrotoluene (TNT) at a similar rate than in the absence of TNT, although global transcriptional analysis using DNA microarrays revealed that TNT exerts some stress. Response to TNT stress is regulated at the transcriptional level, as significant changes in the level of expression of 65 genes were observed. Of these genes, 39 appeared upregulated, and 26 were downregulated. The identity of upregulated genes suggests that P. putida uses two kinds of strategies to overcome TNT toxicity: (i) induction of genes encoding nitroreductases and detoxification‐related enzymes (pnrA, xenD, acpD) and (ii) induction of multidrug efflux pump genes (mexEF/oprN) to reduce intracellular TNT concentrations. Mutants of 13 up‐ and 7 downregulated genes were analysed with regards to TNT toxicity revealing the role of the MexE/MexF/OprN pump and a putative isoquinoline 1‐oxidoreductase in tolerance to TNT. The ORF PP1232 whose transcriptional level did not change in response to TNT affected growth in the presence of nitroaromatic compounds and it was found in a screening of 4000 randomly generated mutants.
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影响因子：
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作者：
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影响因子：
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