Fast-twitch sarcomeric and glycolytic enzyme protein loss in inclusion body myositis.
Fast-twitch sarcomeric and glycolytic enzyme protein loss in inclusion body myositis.
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DOI：
10.1002/mus.21230
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发表时间：
2009-06
期刊：
影响因子：
3.4
通讯作者：
Greenberg, Steven A.
中科院分区：
文献类型：
--
作者：
Parker, Kenneth C.;Kong, Sek Won;Walsh, Ronan J.;Salajegheh, Mohammad;Moghadaszadeh, Behzad;Amato, Anthony A.;Nazareno, Remedios;Lin, Yin Yin;Krastins, Bryan;Sarracino, David A.;Beggs, Alan H.;Pinkus, Jack L.;Greenberg, Steven A.
包涵体肌炎是一种原因不明的骨骼肌炎症性疾病。为了进一步了解这种疾病中组织损伤的性质，我们开发了大规模检测和定量肌肉活检样本中蛋白质的方法，并分析了这些方法产生的蛋白质组学数据以及组织化学、免疫组织化学和微阵列数据。采用液相色谱分离多肽和质谱法对20例炎症性肌病患者（N=17）或无神经肌肉疾病的老年受试者（N=3）的肌肉活检样本进行蛋白质组学研究。另外13个患病样本进行了微阵列研究。采用atp酶组织化学方法对70例神经肌肉疾病患者的肌肉标本进行了检测。较少数量的样本进行了免疫组织化学和免疫印迹研究。质谱研究在每个肌肉样本中鉴定和量化了大约300种不同的蛋白质。在包涵体肌炎和较小程度的多发性肌炎中，通过组织化学、免疫组织化学和免疫印迹证实的蛋白质组学研究表明，尽管转录物水平相对保存，但许多快速收缩特异性结构蛋白和糖酵解酶的丧失。核膜蛋白、免疫球蛋白和两种calpain-3底物的丰度增加。包涵体肌炎肌肉萎缩伴随着快速收缩结构蛋白和糖酵解酶的优先丧失，尤其是糖原去分支酶，而它们各自转录本的丰度相对保存。虽然IBM肌肉萎缩早已被认识到，但这些研究报告了第一个在IBM肌肉中发现的数量减少的特定蛋白质。
Inclusion body myositis (IBM) is an inflammatory disease of skeletal muscle of unknown cause. To further understand the nature of the tissue injury in this disease, we developed methods for large-scale detection and quantitation of proteins in muscle biopsy samples and analyzed proteomic data produced by these methods together with histochemical, immunohistochemical, and microarray data. Twenty muscle biopsy samples from patients with inflammatory myopathies (N=17) or elderly subjects without neuromuscular disease (N=3) were profiled by proteomic studies using liquid chromatographic separation of peptides followed by mass spectrometry. Thirteen of the diseased samples additionally underwent microarray studies. Seventy muscle specimens from patients with a range of neuromuscular disorders were examined by ATPase histochemical methods. Smaller numbers of samples underwent immunohistochemical and immunoblot studies. Mass spectrometric studies identified and quantified approximately 300 total distinct proteins in each muscle sample. In inclusion body myositis and to a lesser extent in polymyositis, proteomic studies confirmed by histochemical, immunohistochemical, and immunoblot studies loss of many fast-twitch specific structural proteins and glycolytic enzymes despite relative preservation of transcript levels. Increased abundance of a nuclear membrane protein, immunoglobulins, and two calpain-3 substrates were present. The atrophy present in inclusion body myositis muscle is accompanied by preferential loss of fast-twitch structural proteins and glycolytic enzymes, particularly glycogen debranching enzyme, with relative preservation of the abundance of their respective transcripts. Although muscle atrophy has long been recognized in IBM, these studies report the first specific proteins identified as reduced in quantity in IBM muscle.
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影响因子：
1.7
作者：
ASKANAS, V;SARKOZI, E;ENGEL, WK
通讯作者：
ENGEL, WK
影响因子：
2.8
作者：
LECLERC, A;TOME, FMS;FARDEAU, M
通讯作者：
FARDEAU, M
DOI：
10.1073/pnas.84.10.3415
发表时间：
1987-05-01
影响因子：
11.1
作者：
NUKINA, N;KOSIK, KS;SELKOE, DJ
通讯作者：
SELKOE, DJ
影响因子：
3.5
作者：
Huang, Yanchao;de Morree, Antoine;van der Maarel, Silvere M.
通讯作者：
van der Maarel, Silvere M.
影响因子：
7
作者：
Ishihama, Y;Oda, Y;Mann, M
通讯作者：
Mann, M