In vivo fate mapping identifies mesenchymal progenitor cells.
In vivo fate mapping identifies mesenchymal progenitor cells.
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DOI：
10.1002/stem.780
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发表时间：
2012-02
期刊：
影响因子：
5.2
通讯作者：
Kalajzic, Ivo
中科院分区：
文献类型：
--
作者：
Grcevic, Danka;Pejda, Slavica;Matthews, Brya G.;Repic, Dario;Wang, Liping;Li, Haitao;Kronenberg, Mark S.;Jiang, Xi;Maye, Peter;Adams, Douglas J.;Rowe, David W.;Aguila, Hector L.;Kalajzic, Ivo
成体间充质祖细胞在再生医学中具有巨大的应用潜力。然而，体内祖细胞及其后代的真实身份尚未精确定义。我们假设表达平滑肌α-肌动蛋白启动子（αSMA）指导的Cre转基因的细胞代表成人骨组织的间充质祖细胞。通过将互补颜色与在谱系成熟阶段激活的转基因相结合，我们表征了表型，并证实了分离的αSMA+细胞从祖细胞进展到完全成熟状态的能力。使用新骨形成模型的体内谱系追踪实验证实了αSMA+细胞的成骨表型。体内标记的SMA 9+细胞的体外分析支持其分化为间充质谱系的潜力。利用骨愈合模型，αSMA+细胞作为纤维软骨和骨骼祖细胞的库。通过骨特异性Col2.3emd转基因的激活来评估骨折愈合过程中αSMA+祖细胞向成熟成骨细胞转变的确认。我们的研究结果提供了一种新的在体内鉴定的确定人口的间充质祖细胞在骨重建和再生的积极作用。
Adult mesenchymal progenitor cells have enormous potential for use in regenerative medicine. However, the true identity of the progenitors in vivo and their progeny has not been precisely defined. We hypothesize that cells expressing a smooth muscle α-actin promoter (αSMA) directed Cre transgene represent mesenchymal progenitors of adult bone tissue. By combining complementary colors in combination with transgenes activating at mature stages of the lineage we characterized the phenotype and confirmed the ability of isolated αSMA+ cells to progress from a progenitor to fully mature state. In vivo lineage tracing experiments using a new bone formation model confirmed the osteogenic phenotype of αSMA+ cells. In vitro analysis of the in vivo labeled SMA9+ cells supported their differentiation potential into mesenchymal lineages. Utilizing a fracture-healing model, αSMA+ cells served as a pool of fibrocartilage and skeletal progenitors. Confirmation of the transition of αSMA+ progenitor cells to mature osteoblasts during fracture healing was assessed by activation of bone specific Col2.3emd transgene. Our findings provide a novel in vivo identification of defined population of mesenchymal progenitor cells with active role in bone remodeling and regeneration.
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影响因子：
5.3
作者：
LEE, TC;CHOW, KL;SCHWARTZ, RJ
通讯作者：
SCHWARTZ, RJ
DOI：
10.1006/bbrc.1997.7124
发表时间：
1997-08-28
影响因子：
3.1
作者：
Feil, R;Wagner, J;Chambon, P
通讯作者：
Chambon, P
影响因子：
64.5
作者：
Sacchetti, Benedetto;Funari, Alessia;Bianco, Paolo
通讯作者：
Bianco, Paolo
影响因子：
20.3
作者：
GRONTHOS, S;GRAVES, SE;SIMMONS, PJ
通讯作者：
SIMMONS, PJ
影响因子：
4
作者：
Gronthos, S;Zannettino, ACW;Simmons, PJ
通讯作者：
Simmons, PJ