Methionine biosynthesis enzyme MoMet2 is required for rice blast fungus pathogenicity by promoting virulence gene expression via reducing 5mC modification.
Methionine biosynthesis enzyme MoMet2 is required for rice blast fungus pathogenicity by promoting virulence gene expression via reducing 5mC modification.
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DOI：
10.1371/journal.pgen.1010927
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发表时间：
2023-09
期刊：
影响因子：
4.5
通讯作者：
中科院分区：
文献类型：
--
作者：
杀真菌剂抗性的出现通过限制已确定的杀真菌剂的可用性和应用而严重威胁作物生产。因此，寻找新的杀菌靶标对植物病害的防治具有重要意义。在这里，我们的特点是一个保守的高丝氨酸O-乙酰转移酶（HOA）的功能，从稻瘟病菌，可以作为候选的抗真菌靶标。编码HOA的MoMET 2和MoCYS 2基因的缺失扰乱了甲硫氨酸和S-腺苷甲硫氨酸（表观遗传修饰的甲基供体）的生物合成，并严重削弱了M的发育和毒力。米。Momet 2突变体在5-甲基胞嘧啶（5 mC）修饰中显著增加，该修饰抑制致病性所需的基因（包括MoGLIK和MoCDH-CYT）的表达。我们进一步证明了靶向MoMET 2和MoCYS 2的宿主诱导的基因沉默（HIGS）有效地控制了水稻稻瘟病。我们的研究揭示了HOA在M.这表明HOA作为新型抗稻瘟病措施的新靶标的潜在可行性。尽管现有的抗真菌剂在防治水稻稻瘟病的有效性，出现这些代理商的抗性需要重新检查真菌病原体，植物真菌的相互作用，和毒力因子。对真菌毒力因子的研究有助于进一步了解真菌的致病机理，并有助于寻找抗真菌药物的作用靶点。我们在这里报告，HOA的真菌原始可以作为一个潜在的目标，抗真菌药物。具体而言，我们发现高丝氨酸O-乙酰基转移酶MoMet 2和MoCys 2在蛋氨酸和S-腺苷蛋氨酸的生物合成中是重要的，蛋氨酸和S-腺苷蛋氨酸对M的生长、发育和毒力至关重要。米。我们进一步证明了MoMet 2介导的5 mC修饰调节基因表达，并且是稻瘟病菌感染所必需的。此外，使用靶向MoMET 2和MoCYS 2基因的宿主诱导的基因沉默（HIGS）提供了针对水稻稻瘟病的保护。总之，这些研究为理解甲硫氨酸合成在真菌致病性中的作用和识别潜在的抗真菌靶点提供了重要的新见解。
The emergence of fungicide resistance severely threatens crop production by limiting the availability and application of established fungicides. Therefore, it is urgent to identify new fungicidal targets for controlling plant diseases. Here, we characterized the function of a conserved homoserine O-acetyltransferase (HOA) from the rice blast fungus Magnaporthe oryzae that could serve as the candidate antifungal target. Deletion of the MoMET2 and MoCYS2 genes encoding HOAs perturbed the biosynthesis of methionine and S-adenyl methionine, a methyl group donor for epigenetic modifications, and severely attenuated the development and virulence of M. oryzae. The ∆Momet2 mutant is significantly increased in 5-methylcytosine (5mC) modification that represses the expression of genes required for pathogenicity, including MoGLIK and MoCDH-CYT. We further showed that host-induced gene silencing (HIGS) targeting MoMET2 and MoCYS2 effectively controls rice blasts. Our studies revealed the importance of HOA in the development and virulence of M. oryzae, which suggests the potential feasibility of HOA as new targets for novel anti-rice blast measurements. Despite the effectiveness of available antifungal agents in combating rice blasts, the emergence of resistance to these agents requires the reexamination of fungal pathogens, plant-fungi interactions, and virulence factors. Characterization of virulence factors is expected to improve the understanding of fungus pathogenesis and to help exploration for an antifungal drug target. We here reported that HOAs of the fungal original could serve as a potential target for antifungal agents. Specifically, we found that homoserine O-acetyltransferases MoMet2 and MoCys2 are important in the biosynthesis of methionine and S-adenyl methionine that are critical for the growth, development, and virulence of M. oryzae. We further demonstrated that MoMet2-mediated 5mC modification regulates gene expression and is required for infection by the rice blast fungus. Additionally, using host-induced gene silencing (HIGS) targeting MoMET2 and MoCYS2 genes provided protection against rice blasts. In all, the studies shed significant new insights in understanding the role of methionine synthesis in fungal pathogenicity and in identifying potential antifungal targets.
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影响因子：
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作者：
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DOI：
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发表时间：
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影响因子：
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