Developing a new treatment for superficial fungal infection using antifungal Collagen-HSAF dressing.
Developing a new treatment for superficial fungal infection using antifungal Collagen-HSAF dressing.
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使用抗真菌胶原蛋白-HSAF 敷料开发治疗浅表真菌感染的新方法
DOI：
10.1002/btm2.10304
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发表时间：
2022-09
影响因子：
7.4
通讯作者：
Yuan, Jin
中科院分区：
文献类型：
--
作者：
Zhong, Jing;Yan, Xiayi;Zuo, Xin;Zhao, Xuan;Yang, Jiahui;Dou, Qin;Peng, Lulu;Zhu, Yuxiang;Xiao, Yichen;Bian, Zeran;He, Dalian;Xu, Qiushuang;Wright, Stephen;Li, Yaoyao;Du, Liangcheng;Wang, Yan;Yuan, Jin
关键词：
真菌病原体是浅表临床感染的常见原因。它们不断增加的耐药性逐渐使现有的抗真菌药物失效。热稳定抗真菌因子（HSAF）是一种结构独特的新型抗真菌天然产物。然而，目前微生物生产者的产量极低，且在水和常见溶剂中的溶解度极差，HSAF 的应用受到阻碍。在这项研究中，我们开发了一种应用 HSAF 治疗浅表真菌感染的有效模式。海洋来源的溶杆菌 YC36 含有 HSAF 生物合成基因簇，我们通过种间信号分子吲哚激活该基因簇。采用高效提取策略，纯度显着提高至 95.3%。扫描电镜图像显示，I型胶原基HSAF（Col-HSAF）具有透明的外观和良好的物理性能，并且HSAF的体外缓释效果维持超过2周。探索了 Col-HSAF 对抗浅表真菌感染的有效治疗浓度，结果发现 Col-HSAF 在烟曲霉角膜炎和皮肤念珠菌病动物中表现出良好的生物相容性、较低的临床评分、轻微的组织学变化和抗真菌能力。总之，Col-HSAF是一种抗真菌试剂，在治疗浅表真菌感染方面具有重要的临床价值。
Fungal pathogens are common causes of superficial clinical infection. Their increasing drug resistance gradually makes existing antifungal drugs ineffective. Heat stable antifungal factor (HSAF) is a novel antifungal natural product with a unique structure. However, the application of HSAF has been hampered by very low yield in the current microbial producers and from extremely poor solubility in water and common solvents. In this study, we developed an effective mode of treatment applying HSAF to superficial fungal infections. The marine‐derived Lysobacter enzymogenes YC36 contains the HSAF biosynthetic gene cluster, which we activated by the interspecific signaling molecule indole. An efficient extraction strategy was used to significantly improve the purity to 95.3%. Scanning electron microscopy images revealed that the Type I collagen‐based HSAF (Col‐HSAF) has a transparent appearance and good physical properties, and the in vitro sustained‐release effect of HSAF was maintained for more than 2 weeks. The effective therapeutic concentration of Col‐HSAF against superficial fungal infection was explored, and Col‐HSAF showed good biocompatibility, lower clinical scores, mild histological changes, and antifungal capabilities in animals with Aspergillus fumigatus keratitis and cutaneous candidiasis. In conclusion, Col‐HSAF is an antifungal reagent with significant clinical value in the treatment of superficial fungal infections.
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抗真菌 HSAF 和产酶溶杆菌类似物中多环系统的生物合成
DOI：
10.1002/anie.201802488
发表时间：
2018-05-22
期刊：
Angewandte Chemie (International ed. in English)
影响因子：
--
作者：
Li Y;Wang H;Liu Y;Jiao Y;Li S;Shen Y;Du L
通讯作者：
Du L
影响因子：
4.4
作者：
Han, Yong;Wang, Yan;Du, Liangcheng
通讯作者：
Du, Liangcheng
影响因子：
16.6
作者：
Li, Yaoyao;Chen, Haotong;Ding, Yanjiao;Xie, Yunxuan;Wang, Haoxin;Cerny, Ronald L.;Shen, Yuemao;Du, Liangcheng
通讯作者：
Du, Liangcheng
影响因子：
4.9
作者：
Yu, Fengan;Zaleta-Rivera, Kathia;Du, Liangcheng
通讯作者：
Du, Liangcheng
影响因子：
2.4
作者：
Tang, B.;Zhao, Y. -C.;Liu, F. -Q.
通讯作者：
Liu, F. -Q.