Immunoreactive atrial natriuretic peptide in the fish heart and blood plasma examined by electron microscopy, immunohistochemistry and radioimmunoassay
Immunoreactive atrial natriuretic peptide in the fish heart and blood plasma examined by electron microscopy, immunohistochemistry and radioimmunoassay
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通过电子显微镜、免疫组织化学和放射免疫分析检测鱼心脏和血浆中的免疫反应性心钠素
作者：
H. Uemura;M. Naruse;T. Hirohama;Sumio Nakamura;Y. Kasuya;T. Aoto
对5种淡水和海水硬骨鱼，以及2种弹性鳃和环口硬骨鱼的心钠素免疫反应性和心肌细胞超微结构进行了观察。在鲤鱼、鳗鱼和康格尔斑点鱼的心房中免疫反应较强，在斑点鱼、大斑鱼、鲑鱼、扁平斑鱼和汉堡石斑鱼的心房中免疫反应较弱，在大花鱼、日本短吻鳗和斑马鱼的心房中免疫反应很弱，而在六头鱼、日本鳗和七鳃鳗的心房中检测不到免疫反应。淡水条件下，心房肌细胞的免疫反应性普遍强于海水鱼。仅在7种动物中检测到心室免疫反应，且总是弱于在心房观察到的。但在超微结构上，所有受检物种的心房和脑室均可见分泌颗粒，前者多于后者。用放射免疫分析法在所有受检物种的血浆提取液和心房室组织中均检测到ANP免疫反应阳性。淡水和海水物种之间的值在统计学上没有显著差异。
SummaryThe immunoreactivity of atrial natriuretic peptide and ultrastructure of cardiocytes were examined in 5 species each of freshwater and seawater teleosts, as well as in 2 species each of elasmobranchs and cyclostomes. Immunoreactivity was strong in the atria of Cyprinus carpio, Anguilla japonica and Conger myriaster, rather weak in atria of Channa maculata, Lepomis macrochirus, Salmo gairdneri, Oplegnathus fasciatus and Eptatretus burgeri, very weak in atria of Pagrus major, Trachurus japonicus and Triakis scyllia, and not detectable in atria of Hexagrammos otakii, Narke japonica and Lampetra japonica. The immunoreactivity of the atrial cardiocytes was generally stronger in freshwater than seawater fish. Ventricular immunoreactivity was detected only in 7 species, always being weaker than that observed in the atrium. Ultrastructurally, however, secretory granules were found in atria and ventricles of all species examined, being more frequent in the former than the latter. By radioimmunoassay, immunoreactive ANP was detected in the extracts of blood plasma and both atrial and ventricular tissues of all species examined. There were no statistically significant differences in the values between freshwater and seawater species.
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DOI：
10.1152/ajpregu.1985.249.3.r348
发表时间：
1985
期刊：
The American journal of physiology
影响因子：
--
作者：
Solomon,R;Taylor,M;Dorsey,D;Silva,P;Epstein,FH
通讯作者：
Epstein,FH
氯离子通道阻滞剂对牛蛙角膜上皮的影响。
DOI：
10.1152/ajpcell.1988.254.4.c519
发表时间：
1988
期刊：
The American journal of physiology
影响因子：
--
作者：
Scheide,JI;Zadunaisky,JA
通讯作者：
Zadunaisky,JA
atriopeptin II 对异斜眼底离体岛盖上皮的影响。
DOI：
10.1152/ajpregu.1988.254.1.r27
发表时间：
1988
期刊：
The American journal of physiology
影响因子：
--
作者：
Scheide,JI;Zadunaisky,JA
通讯作者：
Zadunaisky,JA
人血浆中具有免疫反应性的α-人心房钠尿多肽。
影响因子：
3
作者：
Naruse,M;Naruse,K;Obana,K;Kurimoto,F;Sakurai,H;Honda,T;Higashida,T;Demura,H;Inagami,T;Shizume,K
通讯作者：
Shizume,K
小鼠颌下腺分泌肾素的两种细胞途径的证据。
影响因子：
4.8
作者：
Pratt,RE;Carleton,JE;Roth,TP;Dzau,VJ
通讯作者：
Dzau,VJ