Th17 and Th22 cells in psoriatic arthritis and psoriasis.
Th17 and Th22 cells in psoriatic arthritis and psoriasis.
复制标题
银屑病关节炎和牛皮癣中的Th17和Th22细胞。
DOI：
10.1186/ar4317
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发表时间：
2013-09-26
影响因子：
4.9
通讯作者：
Gaston H
中科院分区：
文献类型：
--
作者：
Benham H;Norris P;Goodall J;Wechalekar MD;FitzGerald O;Szentpetery A;Smith M;Thomas R;Gaston H
本研究的目的是鉴定银屑病（Ps）和银屑病关节炎（PsA）患者外周血（PB）、皮肤、滑液（SF）和滑膜组织（ST）中产生白细胞介素17（IL-17）和白细胞介素22（IL-22）的细胞。采用流式细胞术对11例Ps和12例PsA患者的皮肤和/或SF和PB中产生IL-22和IL-17的细胞进行计数; 15名健康对照的皮肤和PB以及类风湿关节炎（RA）患者的SF用作对照。检测白细胞介素23受体（IL-23 R）和趋化因子受体CCR 4和CCR 6的表达。通过ELISA测量IL-17和IL-22的分泌。ST通过IL-17和IL-22的免疫组织化学染色进行分析。PsA和Ps患者PB中IL-17+和IL-22+ CD 4 + T细胞频率增加。PsA和Ps中IL-17的分泌均显着升高，而PsA中IL-22的分泌高于Ps和健康对照。在Ps和PsA患者中，产生IL-17或IL-22的CD 4+细胞表达IL-23 R的比例较高，IL-17+、CCR 6+和CCR 4 + T细胞的频率升高。在PsA患者中，与PB相比，SF中的CCR 6+和IL-23 R + T细胞数量升高。Ps皮损中IL-17+和IL-22+ CD 4 + T细胞的频率增加。相反，虽然与PB相比，在PsA SF中观察到CD 4 + IL-17+细胞的频率升高，但CD 4 + IL-22+ T细胞的频率较低。而IL-17的表达是等效的PsA，骨关节炎（OA）和RA ST，IL-22的表达是RA高于OA或PsA ST，其中IL-22是明显缺乏。产生IL-17和IL-22的CD 4 + T细胞的频率升高是Ps和PsA的特征。然而，它们在疾病部位的不同分布，包括与皮肤和PB相比SF中IL-22+ CD 4 + T细胞的较低频率，以及ST中IL-22表达的缺乏，表明Th 17和Th 22细胞在Ps和PsA的发病机制中具有共同的以及不同的作用。
The aim of this study was to characterize interleukin 17 (IL-17) and interleukin 22 (IL-22) producing cells in peripheral blood (PB), skin, synovial fluid (SF) and synovial tissue (ST) in patients with psoriasis (Ps) and psoriatic arthritis (PsA). Flow cytometry was used to enumerate cells making IL-22 and IL-17, in skin and/or SF and PB from 11 patients with Ps and 12 patients with PsA; skin and PB of 15 healthy controls and SF from rheumatoid arthritis (RA) patients were used as controls. Expression of the interleukin 23 receptor (IL-23R) and chemokine receptors CCR4 and CCR6 was examined. Secretion of IL-17 and IL-22 was measured by ELISA. ST was analysed by immunohistochemical staining of IL-17 and IL-22. Increased frequencies of IL-17+ and IL-22+ CD4+ T cells were seen in PB of patients with PsA and Ps. IL-17 secretion was significantly elevated in both PsA and Ps, whilst IL-22 secretion was higher in PsA compared to Ps and healthy controls. A higher proportion of the CD4+ cells making IL-17 or IL-22 expressed IL-23R and frequencies of IL-17+, CCR6+ and CCR4+ T cells were elevated in patients with Ps and those with PsA. In patients with PsA, CCR6+ and IL-23R + T cells numbers were elevated in SF compared to PB. Increased frequencies of IL-17+ and IL-22+ CD4+ T cells were demonstrated in Ps skin lesions. In contrast, whilst elevated frequencies of CD4+ IL-17+ cells were seen in PsA SF compared to PB, frequencies of CD4+ IL-22+ T cells were lower. Whereas IL-17 expression was equivalent in PsA, osteoarthritis (OA) and RA ST, IL-22 expression was higher in RA than either OA or PsA ST, in which IL-22 was strikingly absent. Elevated frequencies of IL-17 and IL-22 producing CD4+ T cells were a feature of both Ps and PsA. However their differing distribution at disease sites, including lower frequencies of IL-22+ CD4+ T cells in SF compared to skin and PB, and lack of IL-22 expression in ST suggests that Th17 and Th22 cells have common, as well as divergent roles in the pathogenesis of Ps and PsA.
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DOI：
10.1038/jid.2009.65
发表时间：
2009-09
期刊：
The Journal of investigative dermatology
影响因子：
--
作者：
通讯作者：
--
影响因子：
--
作者：
Ruutu, Merja;Thomas, Gethin;Thomas, Ranjeny
通讯作者：
Thomas, Ranjeny
影响因子：
--
作者：
Kim, Kyoung-Woon;Kim, Hae-Rim;Lee, Sang-Heon
通讯作者：
Lee, Sang-Heon
影响因子：
30.5
作者：
Duhen, Thomas;Geiger, Rebekka;Sallusto, Federica
通讯作者：
Sallusto, Federica
影响因子：
14.2
作者：
Nograles, Kristine E.;Zaba, Lisa C.;Shemer, Avner;Fuentes-Duculan, Judilyn;Cardinale, Irma;Kikuchi, Toyoko;Ramon, Michal;Bergman, Reuven;Krueger, James G.;Guttman-Yassky, Emma
通讯作者：
Guttman-Yassky, Emma