A ROP GTPase signaling pathway controls cortical microtubule ordering and cell expansion in Arabidopsis.
A ROP GTPase signaling pathway controls cortical microtubule ordering and cell expansion in Arabidopsis.
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DOI：
10.1016/j.cub.2009.08.052
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发表时间：
2009-11-17
期刊：
影响因子：
--
通讯作者：
Yang Z
中科院分区：
文献类型：
--
作者：
Fu Y;Xu T;Zhu L;Wen M;Yang Z
皮质微管参与植物生长和形态发生所需的细胞扩张和分裂的空间调控。排列整齐的横向皮质MT促进细胞伸长，限制细胞径向扩张。控制其有序性的分子机制还知之甚少。我们报道了第一个已知的信号通路，它促进了植物皮质MT的组织成平行的阵列，定向垂直于细胞伸长轴。有序的MT局部限制了细胞的扩张，以促进拟南芥叶片拼图形状的铺面细胞中的压痕形成。删除Rho家族GTP酶ROP6使皮质MT随机化，释放了对细胞扩张的局部限制，而ROP6过表达增强了MT的有序性，使细胞的拼图外观变成圆柱形。ROP6直接结合并激活MT相关的RIC1以实现MT排序。ROP6-RIC1途径也影响下胚轴细胞的MT有序性，表明该途径在细胞扩张的空间调节中具有广泛的作用。
Cortical microtubules (MTs) participate in the spatial control of cell expansion and division that is required for plant growth and morphogenesis. Well-ordered transverse cortical MTs promote cell elongation and restrict radial cell expansion. The molecular mechanism controlling their ordering is poorly understood. We report the first known signaling pathway that promotes the organization of cortical MTs into parallel arrays oriented perpendicular to the axis of cell elongation in plants. Well-ordered MTs locally restrict cell expansion to promote indentation formation in the jigsaw puzzle-shaped pavement cells of Arabidopsis leaves. Deleting ROP6, a Rho-family GTPase, randomized cortical MTs and released the localized restriction of cell expansion, whereas ROP6 overexpression enhanced MT ordering, turning the jigsaw puzzle appearance of cells into a cylindrical shape. ROP6 directly binds and activates MT-associated RIC1 to achieve the MT ordering. The ROP6-RIC1 pathway also affects MT ordering of hypocotyl cells, showing a broad role for this pathway in the spatial regulation of cell expansion.
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