Quercetin Preserves Oral Cavity Health by Mitigating Inflammation and Microbial Dysbiosis.
Quercetin Preserves Oral Cavity Health by Mitigating Inflammation and Microbial Dysbiosis.
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DOI：
10.3389/fimmu.2021.774273
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发表时间：
2021
影响因子：
7.3
通讯作者：
Sahingur SE
中科院分区：
文献类型：
--
作者：
Mooney EC;Holden SE;Xia XJ;Li Y;Jiang M;Banson CN;Zhu B;Sahingur SE
未能减轻炎症，加上随之而来的微生物群的变化，驱动骨破坏性牙周炎的发展。槲皮素是一种植物来源的多酚类黄酮，与人类和动物的健康益处有关。我们采用系统的方法，研究了口服槲皮素对宿主炎症反应、口腔微生物组成和牙周病表型的影响。在体内，槲皮素补充减少牙龈细胞因子的表达，炎症细胞浸润和牙槽骨丢失。微生物组分析揭示了槲皮素处理组与赋形剂处理组相比更健康的口腔微生物组成，其特征在于包括肠球菌属、奈瑟氏球菌属和假单胞菌属在内的致病性物种的数量减少以及非致病性链球菌属物种的数量和细菌多样性增加。在体外，槲皮素通过调节口腔细菌和TLR激动剂攻击后的人巨噬细胞中的NF-κB：A20轴来减少炎性细胞因子的产生。总的来说，我们的研究结果表明，槲皮素补充剂通过限制炎症和培养有利于与健康相关的共生微生物群的口腔微环境来促进平衡的牙周组织稳态。这项概念验证研究为转化研究提供了关键证据，以改善整体健康状况。
Failure to attenuate inflammation coupled with consequent microbiota changes drives the development of bone-destructive periodontitis. Quercetin, a plant-derived polyphenolic flavonoid, has been linked with health benefits in both humans and animals. Using a systematic approach, we investigated the effect of orally delivered Quercetin on host inflammatory response, oral microbial composition and periodontal disease phenotype. In vivo, quercetin supplementation diminished gingival cytokine expression, inflammatory cell infiltrate and alveolar bone loss. Microbiome analyses revealed a healthier oral microbial composition in Quercetin-treated versus vehicle-treated group characterized by reduction in the number of pathogenic species including Enterococcus, Neisseria and Pseudomonas and increase in the number of non-pathogenic Streptococcus sp. and bacterial diversity. In vitro, Quercetin diminished inflammatory cytokine production through modulating NF-κB:A20 axis in human macrophages following challenge with oral bacteria and TLR agonists. Collectively, our findings reveal that Quercetin supplement instigates a balanced periodontal tissue homeostasis through limiting inflammation and fostering an oral cavity microenvironment conducive of symbiotic microbiota associated with health. This proof of concept study provides key evidence for translational studies to improve overall health.
DOI：
10.1155/2018/9562803
发表时间：
2018
期刊：
Journal of veterinary medicine
影响因子：
--
作者：
Ye X;Itzoe M;Sarabia-Estrada R;DeTolla L;Tyler BM;Guarnieri M
通讯作者：
Guarnieri M