Microarray Analysis Identifies the Potential Role of Long Non-Coding RNA in Regulating Neuroinflammation during Japanese Encephalitis Virus Infection.
Microarray Analysis Identifies the Potential Role of Long Non-Coding RNA in Regulating Neuroinflammation during Japanese Encephalitis Virus Infection.
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微阵列分析确定长非编码 RNA 在日本脑炎病毒感染期间调节神经炎症中的潜在作用
DOI：
10.3389/fimmu.2017.01237
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发表时间：
2017
影响因子：
7.3
通讯作者：
Cao S
中科院分区：
文献类型：
--
作者：
Li Y;Zhang H;Zhu B;Ashraf U;Chen Z;Xu Q;Zhou D;Zheng B;Song Y;Chen H;Ye J;Cao S
日本脑炎病毒（Japanese encephalitis virus，JEV）是世界范围内流行性脑炎的主要病原。JEV诱导的神经炎症的特征是严重的神经元细胞损伤，伴随着胶质细胞的活化。尽管长链非编码RNA（lncRNA）已成为重要的调控RNA，对多种生物学过程具有重要影响，但lncRNA如何调控乙脑病毒引起的炎症反应尚不清楚。在这里，使用微阵列方法，我们鉴定了618个lncRNA和1,007个mRNA在JEV感染的小鼠脑中差异表达。功能注释分析显示，差异调节的转录本主要参与与宿主免疫和炎症反应相关的各种信号通路。通过构建lncRNA-mRNA共表达网络，筛选出可能参与JEV诱导的炎症反应的lncRNA。此外，两种选定的lncRNA（E52329和N54010）的沉默导致JNK和MKK 4的磷酸化减少，已知它们参与炎症反应。总的来说，我们首先展示了lncRNA在感染JEV的小鼠脑中的转录组景观，并分析了在JEV感染期间差异调节的lncRNA和mRNA的共表达网络。我们的研究结果提供了一个更好的理解宿主对JEV感染的反应，并表明所确定的lncRNA可用作日本脑炎管理的潜在治疗靶点。
Japanese encephalitis virus (JEV) is the leading cause of epidemic encephalitis worldwide. JEV-induced neuroinflammation is characterized by profound neuronal cells damage accompanied by activation of glial cells. Albeit long non-coding RNAs (lncRNAs) have been emerged as important regulatory RNAs with profound effects on various biological processes, it is unknown how lncRNAs regulate JEV-induced inflammation. Here, using microarray approach, we identified 618 lncRNAs and 1,007 mRNAs differentially expressed in JEV-infected mice brain. The functional annotation analysis revealed that differentially regulated transcripts were predominantly involved in various signaling pathways related to host immune and inflammatory responses. The lncRNAs with their potential to regulate JEV-induced inflammatory response were identified by constructing the lncRNA-mRNA coexpression network. Furthermore, silencing of the two selected lncRNAs (E52329 and N54010) resulted in reducing the phosphorylation of JNK and MKK4, which are known to be involved during inflammatory response. Collectively, we first demonstrated the transcriptomic landscape of lncRNAs in mice brain infected with JEV and analyzed the coexpression network of differentially regulated lncRNAs and mRNAs during JEV infection. Our results provide a better understanding of the host response to JEV infection and suggest that the identified lncRNAs may be used as potential therapeutic targets for the management of Japanese encephalitis.
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影响因子：
14.9
作者：
Kambara H;Niazi F;Kostadinova L;Moonka DK;Siegel CT;Post AB;Carnero E;Barriocanal M;Fortes P;Anthony DD;Valadkhan S
通讯作者：
Valadkhan S
影响因子：
7
作者：
Harrow J;Frankish A;Gonzalez JM;Tapanari E;Diekhans M;Kokocinski F;Aken BL;Barrell D;Zadissa A;Searle S;Barnes I;Bignell A;Boychenko V;Hunt T;Kay M;Mukherjee G;Rajan J;Despacio-Reyes G;Saunders G;Steward C;Harte R;Lin M;Howald C;Tanzer A;Derrien T;Chrast J;Walters N;Balasubramanian S;Pei B;Tress M;Rodriguez JM;Ezkurdia I;van Baren J;Brent M;Haussler D;Kellis M;Valencia A;Reymond A;Gerstein M;Guigó R;Hubbard TJ
通讯作者：
Hubbard TJ
DOI：
10.1126/science.1240925
发表时间：
2013-08-16
期刊：
Science (New York, N.Y.)
影响因子：
--
作者：
Carpenter S;Aiello D;Atianand MK;Ricci EP;Gandhi P;Hall LL;Byron M;Monks B;Henry-Bezy M;Lawrence JB;O'Neill LA;Moore MJ;Caffrey DR;Fitzgerald KA
通讯作者：
Fitzgerald KA
影响因子：
10.5
作者：
Cabili, Moran N.;Trapnell, Cole;Rinn, John L.
通讯作者：
Rinn, John L.
影响因子：
5.4
作者：
Chen, CJ;Chen, JH;Raung, SL
通讯作者：
Raung, SL