Backbone and side-chain chemical shift assignments of p50 subunit of NF-κB transcription factor.

Backbone and side-chain chemical shift assignments of p50 subunit of NF-κB transcription factor.
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DOI:
10.1007/s12104-020-09978-z
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发表时间:
2021-04
影响因子:
0.9
通讯作者:
Dyson HJ
Dyson HJ
中科院分区:
生物学4区
文献类型:
--
作者:
Singh A;Dyson HJ

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核因子κB(NF-κB)是一个由5个相关转录因子组成的家族,识别靶基因启动子和增强子区域的κB DNA元件并调节其表达。在这里,我们报告了一套完整的1H,13 C,15 N骨架和侧链共振分配的p50 DNA结合和二聚化结构域的p50同源二聚体形式的NF-κB转录因子。化学位移数据构成了理解p50同二聚体与DNA相互作用机制的第一步。
Nuclear Factor κB (NF-κB) is a family of five related transcription factors that recognize a κB DNA element on the promoter and enhancer regions of target genes and modulate their expression. Here we report a complete set of 1H, 13C, 15N backbone and side chain resonance assignments for the p50 DNA binding and dimerization domains of the p50 homodimer form of the NF-κB transcription factor. The chemical shift data constitute a first step towards understanding the mechanism of interaction of the p50 homodimer with DNA.
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