A role for the cell-wall protein silacidin in cell size of the diatom Thalassiosira pseudonana.

A role for the cell-wall protein silacidin in cell size of the diatom Thalassiosira pseudonana.
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DOI:
10.1038/ismej.2017.100
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发表时间:
2017-11
期刊:
The ISME journal
影响因子:
--
通讯作者:
Mock T
Mock T
中科院分区:
其他
文献类型:
--
作者:
Kirkham AR;Richthammer P;Schmidt K;Wustmann M;Maeda Y;Hedrich R;Brunner E;Tanaka T;van Pée KH;Falciatore A;Mock T

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Diatoms contribute 20% of global primary production and form the basis of many marine food webs. Although their species diversity correlates with broad diversity in cell size, there is also an intraspecific cell-size plasticity owing to sexual reproduction and varying environmental conditions. However, despite the ecological significance of the diatom cell size for food-web structure and global biogeochemical cycles, our knowledge about genes underpinning the size of diatom cells remains elusive. Here, a combination of reverse genetics, experimental evolution and comparative RNA-sequencing analyses enabled us to identify a previously unknown genetic control of cell size in the diatom Thalassiosira pseudonana. In particular, the targeted deregulation of the expression of the cell-wall protein silacidin caused a significant increase in valve diameter. Remarkably, the natural downregulation of the silacidin gene transcript due to experimental evolution under low temperature also correlated with cell-size increase. Our data give first evidence for a genetically controlled regulation of cell size in T. pseudonana and possibly other centric diatoms as they also encode the silacidin gene in their genomes.
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