Continuous monitoring of inflammation biomarkers during simulated cardiopulmonary bypass using a microfluidic immunoassay device - a pilot study.
Continuous monitoring of inflammation biomarkers during simulated cardiopulmonary bypass using a microfluidic immunoassay device - a pilot study.
复制标题
使用微流体免疫测定设备 - 一项试点研究，在模拟心肺旁路期间对炎症生物标志物进行连续监测。
DOI：
10.1111/aor.12021
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发表时间：
2013-01
影响因子：
2.4
通讯作者：
Zahn JD
中科院分区：
文献类型：
--
作者：
Sasso LA;Aran K;Guan Y;Ündar A;Zahn JD
这项工作展示了在体外循环（CPB）过程中使用连续在线监测系统跟踪全身炎症生物标志物。在CPB过程中监测炎症生物标志物的能力将使手术团队能够积极治疗炎症，减少对术后发病率和死亡率的有害影响，从而改善患者的预后。设计了一种微流控装置，它允许微珠免疫测定的单个处理步骤的自动化，以允许抗原浓度的连续跟踪。初步实验表明，微免疫测定产生的结果与标准ELISA产生的结果相当（r=0.98）。此外，该分析与模拟CPB电路的集成已被证明可以对从该电路连续采样的血液中的C3a浓度进行时间跟踪。本文描述了该项目的动机、设计挑战和初步实验结果。
This work demonstrates the use of a continuous online monitoring system for tracking systemic inflammation biomarkers during cardiopulmonary bypass (CPB) procedures. The ability to monitor inflammation biomarkers during CPB will allow surgical teams to actively treat inflammation and reduce harmful effects on postoperative morbidity and mortality, enabling improved patient outcomes. A microfluidic device has been designed which allows automation of the individual processing steps of a microbead immunoassay to allow continuous tracking of antigen concentrations. Preliminary experiments have demonstrated that the results produced by the micro-immunoassay are comparable to results produced from a standard ELISA (r=0.98). Additionally, integration of the assay with a simulated CPB circuit has been demonstrated with temporal tracking of C3a concentrations within blood continuously sampled from the circuit. The presented work describes the motivation, design challenges, and preliminary experimental results of this project.
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影响因子：
2.1
作者：
Bach, F;Grundmann, U;Silomon, M
通讯作者：
Silomon, M
影响因子：
2.4
作者：
Aran, Kiana;Fok, Alex;Uendar, Akif
通讯作者：
Uendar, Akif
影响因子：
2.2
作者：
Kim, Tad;Arnaoutakis, George J.;Beaver, Thomas Mark
通讯作者：
Beaver, Thomas Mark
影响因子：
4.6
作者：
Li, J;Hoschtitzky, A;Redington, AN
通讯作者：
Redington, AN
影响因子：
2.8
作者：
Sasso, Lawrence A.;Undar, Akif;Zahn, Jeffrey D.
通讯作者：
Zahn, Jeffrey D.