The possible role of visceral fat in early pregnancy as a predictor of gestational diabetes mellitus by regulating adipose-derived exosomes miRNA-148 family: protocol for a nested case-control study in a cohort study.
The possible role of visceral fat in early pregnancy as a predictor of gestational diabetes mellitus by regulating adipose-derived exosomes miRNA-148 family: protocol for a nested case-control study in a cohort study.
复制标题
内脏脂肪在妊娠早期通过调节脂肪源性外泌体 miRNA-148 家族作为妊娠糖尿病预测因子的可能作用：队列研究中巢式病例对照研究方案
DOI：
10.1186/s12884-021-03737-1
复制
发表时间：
2021-03-30
影响因子：
3.1
通讯作者：
Wang Q
中科院分区：
文献类型：
--
作者：
Zhang Z;Xu Q;Chen Y;Sui L;Jiang L;Shen Q;Li M;Li G;Wang Q
妊娠期糖尿病（GDM）已成为令人担忧的公共卫生问题。它与不良妊娠结局和产后 2 型糖尿病风险增加有关。孕前体重指数（BMI）、腰围和其他人体测量参数已被提出来预测 GDM。但内脏脂肪厚度可以更好地反映体内脂肪的分布情况，可能更准确地预测GDM的风险。内脏脂肪厚度可能通过调节脂肪源性外泌体miRNA-148家族导致胰岛素抵抗，从而影响GDM的发生。关于内脏脂肪厚度作为 GDM 预测因子及其可能机制的前瞻性队列研究的证据仍然不足。在这项前瞻性队列研究中，我们将招募 3000 名在妊娠 4 至 12 周之间进行首次产前检查的女性。基线社会人口因素和内脏脂肪厚度将分别通过问卷调查和超声波测量进行评估。妊娠20周时，抽取10ml血样，在巢式病例对照研究的基础上提取脂肪源性外泌体miRNA。在妊娠24-28周时进行GDM筛查，并分析患有GDM和未患有GDM的孕妇之间miRNA-148家族的表达。中间分析将用于研究内脏脂肪厚度是否可以通过调节脂肪源性外泌体 miRNA-148 家族来预测 GDM。我们假设内脏脂肪厚度可能通过调节脂肪源性外泌体的 miRNA-148 家族来预测 GDM。该研究结果将有助于进一步阐明GDM的病理生理机制，也将为有效筛查GDM高危孕妇提供技术支撑。在线版本包含可在 10.1186/s12884-021-03737-1 获取的补充材料。
Gestational diabetes mellitus (GDM) has become alarming public health concern. It is associated with adverse pregnancy outcomes and increased risk of postpartum type 2 diabetes. Pre-pregnant body mass index (BMI), waist circumference and other anthropometric parameters have been proposed to predict GDM. However, visceral fat thickness can better reflect the distribution of body fat, and may more accurately predict the risk of GDM. Visceral fat thickness may lead to insulin resistance by regulating the adipose-derived exosomes miRNA-148 family, which affect the development of GDM. Evidence from prospective cohort studies on visceral fat thickness as a predictor of GDM and the possible mechanisms is still insufficient. In this prospective cohort study, we will recruit 3000 women at first antenatal visit between 4 and 12 weeks of gestation. Baseline socio-demographic factors and visceral fat thickness will be assessed by questionnaire form and the ultrasonic measurement, respectively. At 20 weeks of gestation, 10 ml blood samples will be drawn and we will extract adipose-derived exosomes miRNA on the basis of nested case-control study. GDM will be screened at 24–28 weeks’ gestation and the expression of miRNA-148 family between pregnant women with GDM and without GDM will be analyzed. Intermediary analysis will be used to investigate whether visceral fat thickness can predict GDM by regulating adipose-derived exosomes miRNA-148 family. We hypothesized that visceral fat thickness may predict GDM by regulating the miRNA-148 family of adipose-derived exosomes. The findings of the study will assist in further clarifying the pathophysiological mechanism of GDM, it will also provide technical support for effective screening of high-risk pregnant women with GDM. The online version contains supplementary material available at 10.1186/s12884-021-03737-1.
登录
查看更多内容
影响因子：
3.2
作者：
Gao C;Sun X;Lu L;Liu F;Yuan J
通讯作者：
Yuan J
影响因子：
3.7
作者：
Swainson MG;Batterham AM;Tsakirides C;Rutherford ZH;Hind K
通讯作者：
Hind K
影响因子：
15.8
作者：
Daly B;Toulis KA;Thomas N;Gokhale K;Martin J;Webber J;Keerthy D;Jolly K;Saravanan P;Nirantharakumar K
通讯作者：
Nirantharakumar K
影响因子：
81.5
作者：
McIntyre, H. David;Catalano, Patrick;Damm, Peter
通讯作者：
Damm, Peter
脂肪来源的循环 miRNA 调节其他组织中的基因表达。
影响因子：
64.8
作者：
Thomou T;Mori MA;Dreyfuss JM;Konishi M;Sakaguchi M;Wolfrum C;Rao TN;Winnay JN;Garcia-Martin R;Grinspoon SK;Gorden P;Kahn CR
通讯作者：
Kahn CR