Semaphorin3A-neuropilin1 signalling is involved in the generation of cortical interneurons.
Semaphorin3A-neuropilin1 signalling is involved in the generation of cortical interneurons.
复制标题
DOI：
10.1007/s00429-016-1337-3
复制
发表时间：
2017-07
影响因子：
3.1
通讯作者：
Parnavelas JG
中科院分区：
文献类型：
--
作者：
Andrews WD;Barber M;Nemitz M;Memi F;Parnavelas JG
皮质中间神经元主要产生于端脑腹侧的内侧神经节隆起，并在胚胎发育过程中迁移到皮质。这些细胞表达神经纤毛蛋白（Nrp 1和Nrp 2）受体，介导其对化学排斥性3类脑信号蛋白（Sema）配体的反应。我们在这里表明，semaphorins Sema 3A和Sema 3F在邻近皮层中间神经元迁移流的层中以及在纹状体中表达，这表明它们可能在引导这些细胞的整个旅程中发挥作用。对Sema 3A −/−和Sema 3F −/−小鼠在皮质生成过程中的分析表明，Sema 3A的缺乏，而不是Sema 3F，导致皮质中间神经元通过纹状体的异常迁移。在Sema 3A −/−、Nrp 1 −/−和Nrp 2 −/−小鼠的皮质中发现皮质中间神经元数量减少，在Sema 3F −/−、Nrp 1 −/−、Nrp 2 −/−动物中发现分布改变，尤其是在神经纤毛蛋白双突变体中。在Sema 3A −/−和Nrp 1 −/−小鼠中观察到的中间神经元减少是由于它们的祖细胞的增殖活性改变，这通过细胞分裂过程中有丝分裂纺锤体定位和分裂平面角度的变化而突出显示。这些发现指出了Sema 3A-Nrp 1信号在祖细胞动力学和腹侧端脑中间神经元生成中的新作用。本文的在线版本（doi：10.1007/s 00429 -016-1337-3）包含补充材料，可供授权用户使用。
Cortical interneurons are generated predominantly in the medial ganglionic eminence of the ventral telencephalon and migrate to the cortex during embryonic development. These cells express neuropilin (Nrp1 and Nrp2) receptors which mediate their response to the chemorepulsive class 3 semaphorin (Sema) ligands. We show here that semaphorins Sema3A and Sema3F are expressed in layers adjacent to cortical interneuron migratory streams as well as in the striatum, suggesting they may have a role in guiding these cells throughout their journey. Analysis of Sema3A −/− and Sema3F −/− mice during corticogenesis showed that absence of Sema3A, but not Sema3F, leads to aberrant migration of cortical interneurons through the striatum. Reduced number of cortical interneurons was found in the cortex of Sema3A −/−, Nrp1 −/− and Nrp2 −/− mice, as well as altered distribution in Sema3F −/−, Nrp1 −/−, Nrp2 −/− animals and especially in neuropilin double mutants. The observed decrease in interneurons in Sema3A −/− and Nrp1 −/− mice was due to altered proliferative activity of their progenitors highlighted by changes in their mitotic spindle positioning and angle of cleavage plane during cell division. These findings point to a novel role for Sema3A–Nrp1 signalling in progenitor cell dynamics and in the generation of interneurons in the ventral telencephalon. The online version of this article (doi:10.1007/s00429-016-1337-3) contains supplementary material, which is available to authorized users.
登录
查看更多内容
DOI：
10.1002/cne.23806
发表时间：
2016-02-15
期刊：
The Journal of comparative neurology
影响因子：
--
作者：
Andrews WD;Davidson K;Tamamaki N;Ruhrberg C;Parnavelas JG
通讯作者：
Parnavelas JG
影响因子：
3.5
作者：
Cariboni, Anna;Davidson, Kathryn;Ruhrberg, Christiana
通讯作者：
Ruhrberg, Christiana
影响因子：
25
作者：
Hansen, David V.;Lui, Jan H.;Flandin, Pierre;Yoshikawa, Kazuaki;Rubenstein, John L.;Alvarez-Buylla, Arturo;Kriegstein, Arnold R.
通讯作者：
Kriegstein, Arnold R.
影响因子：
5.3
作者：
Bagnard, D;Vaillant, C;Thomasset, N
通讯作者：
Thomasset, N
影响因子：
16.2
作者：
Giger, RJ;Cloutier, JF;Geppert, M
通讯作者：
Geppert, M