Ion-Pairing Chromatography and Amine Derivatization Provide Complementary Approaches for the Targeted LC-MS Analysis of the Polar Metabolome.
Ion-Pairing Chromatography and Amine Derivatization Provide Complementary Approaches for the Targeted LC-MS Analysis of the Polar Metabolome.
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DOI：
10.1021/acs.jproteome.2c00030
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发表时间：
2022-06-03
影响因子：
4.4
通讯作者：
Bundy, Jacob G.
中科院分区：
文献类型：
--
作者：
Sagi-Kiss, Virag;Li, Yufeng;Carey, Matthew R.;Grover, Sarah J.;Siems, Karsten;Cirulli, Francesca;Berry, Alessandra;Musillo, Chiara;Wilson, Ian D.;Want, Elizabeth J.;Bundy, Jacob G.
关键词：
液相色谱-质谱联用技术是代谢组学的关键技术。反相液相色谱法（RPLC）是一种非常广泛使用的分离步骤，但通常对高极性代谢物的保留较差。在这里，我们评估了两种替代方法的组合，以提高保留极性代谢产物的基础上6-氨基喹啉-N-羟基琥珀酰亚胺氨基甲酸酯衍生化胺基团，和离子配对色谱法（IPC）使用三丁胺作为离子配对剂保留酸。我们比较了这两种方法的反相高效液相色谱法，也彼此，有针对性的分析，使用三重四极杆质谱仪，适用于图书馆的ca。500种极性代谢物。IPC和衍生化在覆盖范围方面是互补的：结合起来，它们将具有良好保留的代谢物比例提高到91%，而单独使用RPLC仅为39%。通过分析一组用多酚化合物蛇葡萄素处理过的老年雄性和雌性小鼠的肝脏提取物，对这种组合方法进行了评估。不仅检测到许多显著变化的代谢物，而且还可以表明蛇葡萄素处理和性别之间存在明显的相互作用，因为雄性和雌性的代谢物变化方向相反。
Liquid chromatography coupled to mass spectrometry is a key metabolomics/metabonomics technology. Reversed-phase liquid chromatography (RPLC) is very widely used as a separation step, but typically has poor retention of highly polar metabolites. Here, we evaluated the combination of two alternative methods for improving retention of polar metabolites based on 6-aminoquinoloyl-N-hydroxysuccinidimyl carbamate derivatization for amine groups, and ion-pairing chromatography (IPC) using tributylamine as an ion-pairing agent to retain acids. We compared both of these methods to RPLC and also to each other, for targeted analysis using a triple-quadrupole mass spectrometer, applied to a library of ca. 500 polar metabolites. IPC and derivatization were complementary in terms of their coverage: combined, they improved the proportion of metabolites with good retention to 91%, compared to just 39% for RPLC alone. The combined method was assessed by analyzing a set of liver extracts from aged male and female mice that had been treated with the polyphenol compound ampelopsin. Not only were a number of significantly changed metabolites detected, but also it could be shown that there was a clear interaction between ampelopsin treatment and sex, in that the direction of metabolite change was opposite for males and females.
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影响因子：
7.7
作者：
Markley JL;Brüschweiler R;Edison AS;Eghbalnia HR;Powers R;Raftery D;Wishart DS
通讯作者：
Wishart DS
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通讯作者：
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影响因子：
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作者：
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通讯作者：
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