Characterization of the transcriptome, nucleotide sequence polymorphism, and natural selection in the desert adapted mouse Peromyscus eremicus.
Characterization of the transcriptome, nucleotide sequence polymorphism, and natural selection in the desert adapted mouse Peromyscus eremicus.
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作者：
MacManes MD;Eisen MB
由于高温和干旱的直接影响，沙漠被认为是最恶劣的环境之一，尤其是对生活在沙漠中的哺乳动物来说。鉴于渗透调节对这些动物来说是具有挑战性的，失败会导致死亡，因此应该观察到与维持水和溶质平衡相关的基因存在强选择。一种这样的动物，Peromyscus eremicus，原产于美国西南部的沙漠地区，可能一生都不摄入口服液。作为了解这种表型的遗传学基础的第一步，我们提出了P. eremicus转录组的表征。我们分析了来自单个个体的四个组织（肾脏、肝脏、大脑、睾丸），并补充了来自另外15只动物的群体水平肾脏转录组测序。根据Tajima’s D.的估计，我们确定了一组经过纯化和平衡选择的转录本。此外，我们使用分支位点测试确定了一个转录本- slc2a9，可能与荒漠渗透调节有关，在P. eremicus中相对于一组相关的非沙漠啮齿动物进行了增强选择。
As a direct result of intense heat and aridity, deserts are thought to be among the most harsh of environments, particularly for their mammalian inhabitants. Given that osmoregulation can be challenging for these animals, with failure resulting in death, strong selection should be observed on genes related to the maintenance of water and solute balance. One such animal, Peromyscus eremicus, is native to the desert regions of the southwest United States and may live its entire life without oral fluid intake. As a first step toward understanding the genetics that underlie this phenotype, we present a characterization of the P. eremicus transcriptome. We assay four tissues (kidney, liver, brain, testes) from a single individual and supplement this with population level renal transcriptome sequencing from 15 additional animals. We identified a set of transcripts undergoing both purifying and balancing selection based on estimates of Tajima’s D. In addition, we used the branch-site test to identify a transcript—Slc2a9, likely related to desert osmoregulation—undergoing enhanced selection in P. eremicus relative to a set of related non-desert rodents.
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影响因子：
4.5
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Aubry S;Kelly S;Kümpers BM;Smith-Unna RD;Hibberd JM
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