miR-18a-5p Targets FBP1 to Promote Proliferation, Migration, and Invasion of Liver Cancer Cells and Inhibit Cell Apoptosis.
miR-18a-5p Targets FBP1 to Promote Proliferation, Migration, and Invasion of Liver Cancer Cells and Inhibit Cell Apoptosis.
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miR-18a-5p靶向FBP1，以促进肝癌细胞的增殖，迁移和侵袭并抑制细胞凋亡。
DOI：
10.1155/2021/3334065
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发表时间：
2021
影响因子：
--
通讯作者：
Ren X
中科院分区：
文献类型：
--
作者：
Gao S;Zhu D;Zhu J;Shen L;Zhu M;Ren X
肝癌是最具侵袭性的恶性肿瘤之一。了解肝癌细胞的分子机制对制定新的治疗方案具有重要意义。研究表明，FBP 1是一种癌症抑制基因。为研究FBP 1在肝癌细胞中的作用机制，采用生物信息学方法分析其在肝癌组织中的表达。还进行了生存分析。利用starBase数据库对FBP 1的上游调控基因进行预测。采用双荧光素酶法检测它们的靶向性。分别采用qRT-PCR和western blot检测肝癌细胞FBP 1 mRNA和蛋白的表达水平。通过CCK-8测定分析细胞活力。Transwell法检测细胞的迁移和侵袭能力。流式细胞仪检测肝癌细胞凋亡。结果显示，FBP 1在肝癌组织和细胞中表达下调。FBP 1低表达与患者预后不良相关。miR-18 a-5 p可抑制FBP 1的表达。FBP 1过表达可抑制肝癌细胞的生长，促进细胞凋亡。过表达miR-18 a-5 p可促进肝癌细胞的生长，抑制细胞凋亡。然而，同时过表达FBP 1可以逆转这种效应。miR-18 a-5 p和FBP 1有望成为肝癌治疗的候选药物。
Liver cancer is one of the most aggressive malignant tumors. It is significant to understand the molecular mechanism of liver cancer cells to develop new treatment plans. Studies have identified that FBP1 serves as a cancer inhibitor gene. To research the effect mechanism of FBP1 in liver cancer cells, bioinformatics analysis was performed to study its expression in liver cancer tissue. Survival analysis was also performed. Moreover, starBase database was applied to predict upstream regulatory genes of FBP1. Dual-luciferase assay was performed to testify their targeted relationship. The mRNA and protein expression levels of FBP1 in liver cancer cells were detected by qRT-PCR and western blot, respectively. Cell viability was analyzed by CCK-8 assay. The migratory and invasive abilities of cells were analyzed by Transwell assay. The apoptosis of liver cancer cells was detected by flow cytometry. The results showed that the expression of FBP1 was downregulated in liver cancer tissue and cells. FBP1 low expression was correlated with the poor prognosis of patients. miR-18a-5p could inhibit FBP1 expression. Overexpression of FBP1 could inhibit the progression of liver cancer cells and promote cell apoptosis. Overexpressing miR-18a-5p could promote the progression of liver cancer cells and inhibit cell apoptosis. However, overexpressing FBP1 simultaneously could reverse the effect. miR-18a-5p and FBP1 are expected to be candidates for liver cancer treatment.
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影响因子：
4
作者：
Zhang, Delin;Li, Zhu;Huang, Jianzhao
通讯作者：
Huang, Jianzhao
影响因子：
7.5
作者：
Li, Yuanhang;Bai, Weijun;Zhang, Jianjun
通讯作者：
Zhang, Jianjun
影响因子：
2.8
作者：
Liu, Chao;Xu, Dingwei;Huang, Jie
通讯作者：
Huang, Jie
非小细胞肺癌中FBP1基因甲基化的重要性。
影响因子：
--
作者：
Dong Y;Huaying S;Danying W;Chihong Z;Ruibin J;Xiaojiang S;Jianguo F
通讯作者：
Jianguo F
影响因子：
2.9
作者：
Lu C;Peng K;Guo H;Ren X;Hu S;Cai Y;Han Y;Ma L;Xu P
通讯作者：
Xu P