miR-18a-5p promotes cell invasion and migration of osteosarcoma by directly targeting IRF2.
miR-18a-5p promotes cell invasion and migration of osteosarcoma by directly targeting IRF2.
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DOI：
10.3892/ol.2018.9032
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发表时间：
2018-09
期刊：
影响因子：
2.9
通讯作者：
Xu P
中科院分区：
文献类型：
--
作者：
Lu C;Peng K;Guo H;Ren X;Hu S;Cai Y;Han Y;Ma L;Xu P
越来越多的研究表明microRNAs（miRNAs）参与了包括骨肉瘤（OS）在内的多种人类肿瘤的发生发展。特别是microRNA-18 a-5 p（miR-18 a-5 p）与人类肿瘤的发生、发展和临床结局相关。因此，我们研究了miR-18 a-5 p在OS中的功能。RT-qPCR结果显示，miR-18 a-5 p在OS组织和细胞系（MG-63和Saos-2）中表达显著上调。通过Transwell实验发现miR-18 a-5 p过表达可显著促进MG-63细胞的迁移和侵袭。此外，荧光素酶报告基因分析表明干扰素调节因子（IRF）2是miR-18 a-5 p的直接靶点。IRF 2在MG-63和Saos-2细胞系中下调。此外，Transwell分析显示，IRF 2的敲除促进MG-63细胞的迁移和侵袭。结论：miR-18 a-5 p通过抑制IRF 2的表达，促进OS细胞的侵袭和迁移。因此，miR-18 a-5 p有可能成为OS诊断和治疗的潜在靶点。
An increasing number of studies have suggested that microRNAs (miRNAs) are involved in the progress of many human cancers including osteosarcoma (OS). Especially, microRNA-18a-5p (miR-18a-5p) has been reported to associate with the occurrence, development and clinical outcomes of human cancers. Therefore, we investigated the functions of miR-18a-5p in OS. Reverse transcription-quantitative PCR (RT-qPCR) showed that miR-18a-5p was significantly upregulated in OS tissues and cell lines (MG-63 and Saos-2). The overexpression of miR-18a-5p was found to significantly promote cell migration and invasion in MG-63 cells via Transwell assay. Moreover, luciferase reporter assays indicated that interferon regulatory factor (IRF)2 was a direct target of miR-18a-5p. IRF2 was downregulated in MG-63 and Saos-2 cell lines. Furthermore, Transwell analysis showed that the knockout of IRF2 promoted cell migration and invasion in MG-63 cells. Carcinogenesis of miR-18a-5p was reversed by the overexpression of IRF2 in OS. In conclusion, miR-18a-5p promoted the invasion and migration of OS cells through inhibiting IRF2 expression. Thus, miR-18a-5p might act as a potential target for the diagnosis and treatment of OS in the future.
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