Allelic variation in TLR4 is linked to resistance to Salmonella Enteritidis infection in chickens
Allelic variation in TLR4 is linked to resistance to Salmonella Enteritidis infection in chickens
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TLR4 等位基因变异与鸡对肠炎沙门氏菌感染的抵抗力有关
DOI：
10.3382/ps/pex010
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发表时间：
2017-07
期刊：
影响因子：
4.4
通讯作者：
Jie Wen
中科院分区：
文献类型：
--
作者：
Peng Li;Huihua Wang;Xingwang Zhao;Zhongyong Gou;Ranran Liu;Yongmei Song;Qinghe Li;Maiqing Zheng;Huanxian Cui;Nadia Everaert;Guiping Zhao;Jie Wen
肠炎沙门氏菌 (SE) 是一种食源性病原体，会对动物和人类健康产生负面影响。 TLR4基因的多态性可能影响Toll样受体4（TLR4）对细菌脂多糖（LPS）的识别，导致宿主对病原感染的抵抗力存在差异。本研究调查了十个鸡品种中鸡 TLR4 (ChTLR4) 的多态性位点、TLR4 突变结构的静电势，以及无特定病原体 (SPF) 白来航鸡中等位基因变异与 SE 感染存活率之间的连锁分析。在鸡品种中总共发现了 19 个单核苷酸多态性 (SNP)，其中 10 个是新的。 ChTLR4 基因的胞外域中证实了七个新鉴定的氨基酸变体（C68G、G674A、G782A、A896T、T959G、T986A 和 A1104C）和先前报道的重要突变（G247A、G1028A、C1147T 和 A1832G）。通过同源建模建立的 TLR4 胞外域表面静电势在 Glu83Lys (G247A)、Arg298Ser (A896T)、Ser368Arg (A1104C) 和 Gln611Arg (A1832G) 取代处观察到显着变化。连锁分析表明，TLR4 基因的一个多态性位点 G247A 在所有受检品种中都很常见，与 SPF 白来航雏鸡对 SE 的抵抗力增强显着相关（对数秩 P 值=0.04）。 A1832G SNP 的基因型没有显示出统计学上显着的生存差异。这项研究提供了第一个直接证据，证明 ChTLR4 中的 G247A 替代与肠炎沙门氏菌耐药性增加相关。
Salmonella Enteritidis (SE) is a foodborne pathogen that negatively affects both animal and human health. Polymorphisms of the TLR4 gene may affect recognition by Toll-like receptor 4 (TLR4) of bacterial lipopolysaccharide (LPS), leading to differences in host resistance to pathogenic infections. The present study has investigated polymorphic loci of chicken TLR4 (ChTLR4) in ten chicken breeds, electrostatic potentials of mutant structures of TLR4, and a linkage analysis between allelic variation and survival ratio to infection with SE in specific-pathogenfree (SPF) White Leghorns. A total of 19 Single Nucleotide Polymorphisms (SNPs), of which 10 were novel, were found in chicken breeds. Seven newly identified amino acid variants (C68G, G674A, G782A, A896T,.T959G, T986A, and A1104C) and previously reported important mutations (G247A, G1028A, C1147T, and A1832G) were demonstrated in the extracellular domain of the ChTLR4 gene. Significant changes in surface electrostatic potential of the ectodomain of TLR4, built by homology modeling, were observed at the Glu83Lys (G247A), Arg298Ser (A896T), Ser368Arg (A1104C), and Gln611Arg (A1832G) substitutions. Linkage analysis showed that one polymorphic locus.G247A of TLR4 gene, common in all breeds examined, was significantly associated with increased resistance to SE in SPF White Leghorns chicks (log-rank P-value =0.04). The genotypes from A1832G SNPs did not show statistically significant survival differences. This study has provided the first direct evidence that G247A substitution in ChTLR4 is associated with increased resistance to Salmonella Enteritidis.
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影响因子：
8
作者：
COLOVOS, C;YEATES, TO
通讯作者：
YEATES, TO
影响因子：
2.9
作者：
P. Wigley
通讯作者：
P. Wigley
影响因子：
3.7
作者：
Gou Z;Liu R;Zhao G;Zheng M;Li P;Wang H;Zhu Y;Chen J;Wen J
通讯作者：
Wen J
影响因子：
3.1
作者：
Leveque, G;Forgetta, V;Malo, D
通讯作者：
Malo, D
影响因子：
3.7
作者：
Calenge F;Beaumont C
通讯作者：
Beaumont C