Molecular Evolution of Slow and Quick Anion Channels (SLACs and QUACs/ALMTs).
Molecular Evolution of Slow and Quick Anion Channels (SLACs and QUACs/ALMTs).
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DOI：
10.3389/fpls.2012.00263
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发表时间：
2012
影响因子：
5.6
通讯作者：
Geiger D
中科院分区：
文献类型：
--
作者：
Dreyer I;Gomez-Porras JL;Riaño-Pachón DM;Hedrich R;Geiger D
大约25年前进行的电生理分析在保护细胞的质膜上发现了两种阴离子通道。一种通道对膜电压的变化反应缓慢，而另一种通道反应迅速。因此，它们被命名为SLAC（慢阴离子通道）和QUAC（快阴离子通道）。最近，在模式植物拟南芥中发现了两种不同阴离子电流成分的基因SLAC1和QUAC1/ALMT12。基因产物在非洲爪蟾卵母细胞中的表达证实了电流的快慢动力学。本文综述了植物中快慢阴离子通道的研究进展，并分析了ALMT/ quac样和slac样阴离子通道的分子进化过程。我们发现了允许筛选这些通道类型数据库的指纹图谱，并能够在32种植物的完全测序基因组中鉴定出192个（177个非冗余）slac样蛋白和422个（402个非冗余）ALMT/ quac样蛋白。系统发育分析为这些通道类型的分子演化提供了新的见解。我们还将序列比对和聚类与蛋白质特征的预测相结合，从而确定了slac1样通道中已知的保守磷酸化位点以及尚未实验证实的潜在位点。使用类似的策略来分析ALMT/ quac样通道的亲水性，我们提出了一种带有额外跨膜区域的改进拓扑结构，该区域集成了这些膜蛋白的结构和功能。我们的研究结果表明，交叉引用系统发育分析与位置特异性蛋白质特性和功能数据可能是基因组研究方法的一个非常强大的工具。
Electrophysiological analyses conducted about 25 years ago detected two types of anion channels in the plasma membrane of guard cells. One type of channel responds slowly to changes in membrane voltage while the other responds quickly. Consequently, they were named SLAC, for SLow Anion Channel, and QUAC, for QUick Anion Channel. Recently, genes SLAC1 and QUAC1/ALMT12, underlying the two different anion current components, could be identified in the model plant Arabidopsis thaliana. Expression of the gene products in Xenopus oocytes confirmed the quick and slow current kinetics. In this study we provide an overview on our current knowledge on slow and quick anion channels in plants and analyze the molecular evolution of ALMT/QUAC-like and SLAC-like channels. We discovered fingerprints that allow screening databases for these channel types and were able to identify 192 (177 non-redundant) SLAC-like and 422 (402 non-redundant) ALMT/QUAC-like proteins in the fully sequenced genomes of 32 plant species. Phylogenetic analyses provided new insights into the molecular evolution of these channel types. We also combined sequence alignment and clustering with predictions of protein features, leading to the identification of known conserved phosphorylation sites in SLAC1-like channels along with potential sites that have not been yet experimentally confirmed. Using a similar strategy to analyze the hydropathicity of ALMT/QUAC-like channels, we propose a modified topology with additional transmembrane regions that integrates structure and function of these membrane proteins. Our results suggest that cross-referencing phylogenetic analyses with position-specific protein properties and functional data could be a very powerful tool for genome research approaches in general.
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DOI：
10.1093/bioinformatics/btq224
发表时间：
2010-08-01
期刊：
Bioinformatics (Oxford, England)
影响因子：
--
作者：
Katoh K;Toh H
通讯作者：
Toh H
影响因子：
11.4
作者：
HEDRICH, R;BUSCH, H;RASCHKE, K
通讯作者：
RASCHKE, K
DOI：
10.1073/pnas.0912021106
发表时间：
2009-12-15
影响因子：
11.1
作者：
Geiger, Dietmar;Scherzer, Soenke;Hedrich, Rainer
通讯作者：
Hedrich, Rainer
DOI：
10.1073/pnas.0500146102
发表时间：
2005-03-15
影响因子：
11.1
作者：
Levchenko, V;Konrad, KR;Hedrich, R
通讯作者：
Hedrich, R
影响因子：
7.2
作者：
Kovermann, Peter;Meyer, Stefan;Martinoia, Enrico
通讯作者：
Martinoia, Enrico