β-adrenoceptor regulates miRNA expression in rat heart.
β-adrenoceptor regulates miRNA expression in rat heart.
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β-肾上腺素受体调节大鼠心脏中 miRNA 的表达
DOI：
10.12659/msm.883263
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发表时间：
2012-08
期刊：
影响因子：
--
通讯作者：
Lu Y
中科院分区：
文献类型：
--
作者：
Hou Y;Sun Y;Shan H;Li X;Zhang M;Zhou X;Xing S;Sun H;Chu W;Qiao G;Lu Y
microRNA（miRNAs）是一类18-25个核苷酸的非编码RNA，在转录后调节基因表达，参与多种生理和病理过程。β-肾上腺素能信号通路在心脏功能调节中起着重要作用。本研究旨在探讨β-肾上腺素受体激活或抑制条件下大鼠心脏miRNAs的表达谱及其功能意义。用异丙肾上腺素（ISO）或普萘洛尔（PRO）处理Wistar大鼠，测定其血流动力学参数以评价心功能。通过miRNA微阵列分析miRNA表达，并通过实时定量逆转录PCR（real-time qRT-PCR）确认。异丙肾上腺素（ISO，β-肾上腺素受体激活剂）和普萘洛尔（PRO，β-肾上腺素受体抑制剂）诱导差异miRNA表达谱。在检测的349个miRNAs中，ISO组有43个上调，9个下调，而PRO组有5个上调，28个下调。在PRO组和ISO组中的这些改变的miRNA中，11个是心脏丰富的，11个在PRO组和ISO组之间显示相反的谱。在ISO组中，公认的抗肥大miRNAs miR-1、miR-21和miR-27 b以及促肥大miRNAs miR-22、miR-24、miR-199 a、miR-212和miR-214上调。在PRO组中，促肥大miRNA miR-30 c上调，而miR-212下调。β-肾上腺素能受体的干预改变了miRNA的表达谱，miRNA可能参与了β-肾上腺素能受体信号通路。心肌细胞肥大是促肥大和抗肥大调节之间的平衡过程，至少涉及miRNA的参与。
MicroRNAs (miRNAs) are noncoding RNAs of 18–25 nucleotides that post-transcriptionally regulate gene expression and are involved in a wide range of physiological and pathological conditions. The β-adrenergic signaling pathway plays a fundamental role in regulation of heart function. The present study was designed to investigate the expression profile of miRNAs and functional implications under conditions of β-adrenoceptor activation or inhibition in rat heart. Hemodynamic parameters were measured to assess heart function in Wistar rats treated with isoproterenol (ISO) or propranolol (PRO). miRNA expression was analyzed by miRNA Microarray and confirmed by real-time quantitative reverse transcription PCR (real-time qRT-PCR). Isoproterenol (ISO, a β-adrenoceptor activator) and propranolol (PRO, a β-adrenoceptor inhibitor) induced differential miRNA expression profiles. Out of 349 miRNAs measured, 43 were upregulated and nine downregulated in the ISO group, while five miRNAs were upregulated and 28 downregulated in PRO group. Among these altered miRNAs in both PRO and ISO groups, 11 were cardiac abundant and 11 showed opposite profiles between the PRO and ISO groups. The recognized anti-hypertrophic miRNAs miR-1, miR-21 and miR-27b, and the pro-hypertrophic miRNAs miR-22, miR-24, miR-199a, miR-212 and miR-214, were upregulated in the ISO group. In the PRO group, pro-hypertrophic miRNA miR-30c was upregulated, whereas miR-212 was downregulated. β-adrenoceptor intervention alters miRNA expression profile, and miRNAs may be involved in the β-adrenoceptor signaling pathway. Cardiomyocyte hypertrophy is a balanced process between pro-hypertrophic and anti-hypertrophic regulation and involves, at the very least, miRNA participation.
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DOI：
10.1073/pnas.0602266103
发表时间：
2006-05-02
影响因子：
11.1
作者：
Costinean, S;Zanesi, N;Croce, CM
通讯作者：
Croce, CM
影响因子：
20.1
作者：
Divakaran V;Mann DL
通讯作者：
Mann DL
影响因子：
4.8
作者：
Müller, FU;Lewin, G;Schmitz, W
通讯作者：
Schmitz, W
影响因子：
5
作者：
Jentzsch, Claudia;Leierseder, Simon;Engelhardt, Stefan
通讯作者：
Engelhardt, Stefan
影响因子：
11.4
作者：
Lee, Y;Kim, M;Kim, VN
通讯作者：
Kim, VN