OsNHX5-mediated pH homeostasis is required for post-Golgi trafficking of seed storage proteins in rice endosperm cells
OsNHX5-mediated pH homeostasis is required for post-Golgi trafficking of seed storage proteins in rice endosperm cells
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OsNHX5 介导的 pH 稳态是水稻胚乳细胞中种子储存蛋白的后高尔基体运输所必需的
DOI：
10.1186/s12870-019-1911-y
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发表时间：
2019-07
影响因子：
5.3
通讯作者：
Wan Jianmin
中科院分区：
文献类型：
--
作者：
Zhu Jianping;Ren Yulong;Wang Yunlong;Liu Feng;Teng Xuan;Zhang Yuanyan;Duan Erchao;Wu Mingming;Zhong Mingsheng;Hao Yuanyuan;Zhu Xiaopin;Lei Jie;Wang Yongfei;Yu Yanfang;Pan Tian;Bao Yiqun;Wang Yihua;Wan Jianmin
背景作为水稻种子中的主要储存蛋白，谷蛋白在内质网（ER）中合成为谷蛋白原，并转运至称为PBII（蛋白体II）的蛋白质储存液泡（PSV），在那里它们被液泡加工酶裂解成成熟形式。然而，谷蛋白运输的分子机制在很大程度上尚不清楚。结果在本研究中，我们报道了一种水稻突变体，名为谷蛋白前体积累6（gpa6），它异常地积累了大量的谷蛋白前体。细胞学分析表明，ingpa6 胚乳细胞的谷蛋白原发生错误分选，导致质外体中出现致密囊泡 (DV) 并形成旁壁体 (PMB)，因此观察到较小的 PBII。突变基因ingpa6被发现编码Na+/H+反向转运蛋白OsNHX5。OsNHX5在分析的所有组织中都有表达，并且其表达水平远高于其最接近的旁系同源物OsNHX6。 OsNHX5 蛋白共定位于烟叶表皮细胞中的高尔基体、反式高尔基体网络 (TGN) 和液泡前室 (PVC)。体内 pH 测量表明，ingpa6 的高尔基体、TGN 和 PVC 的管腔变得更加酸性。结论我们的结果证明了 OsNHX5 在调节内膜管腔 pH 值方面的重要作用，这对于水稻种子储存蛋白的运输至关重要。
BackgroundAs the major storage protein in rice seeds, glutelins are synthesized at the endoplasmic reticulum (ER) as proglutelins and transported to protein storage vacuoles (PSVs) called PBIIs (Protein body IIs), where they are cleaved into mature forms by the vacuolar processing enzymes. However, the molecular mechanisms underlying glutelin trafficking are largely unknown.ResultsIn this study, we report a rice mutant, namedglutelin precursor accumulation6(gpa6), which abnormally accumulates massive proglutelins. Cytological analyses revealed that ingpa6endosperm cells, proglutelins were mis-sorted, leading to the presence of dense vesicles (DVs) and the formation paramural bodies (PMBs) at the apoplast, consequently, smaller PBII were observed. Mutated gene ingpa6was found to encode a Na+/H+antiporter,OsNHX5.OsNHX5is expressed in all tissues analyzed, and its expression level is much higher than its closest paralogOsNHX6. The OsNHX5 protein colocalizes to the Golgi, thetrans-Golgi network (TGN) and the pre-vacuolar compartment (PVC) in tobacco leaf epidermal cells. In vivo pH measurements indicated that the lumens of Golgi, TGN and PVC became more acidic ingpa6.ConclusionsOur results demonstrated an important role of OsNHX5 in regulating endomembrane luminal pH, which is essential for seed storage protein trafficking in rice.
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影响因子：
5.6
作者：
Chanroj S;Wang G;Venema K;Zhang MW;Delwiche CF;Sze H
通讯作者：
Sze H
影响因子：
5.3
作者：
通讯作者：
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谷蛋白前体累积3编码对水稻胚乳液泡蛋白分选至关重要的后高尔基体囊泡运输调节因子
影响因子：
11.6
作者：
Ren, Yulong;Wang, Yihua;Wan, Jianmin
通讯作者：
Wan, Jianmin
影响因子：
4.9
作者：
Chen, Songbiao;Tao, Lizen;Wang, Guo-Liang
通讯作者：
Wang, Guo-Liang
DOI：
10.1101/pdb.prot4995
发表时间：
2008-04-01
期刊：
CSH protocols
影响因子：
--
作者：
Waadt, Rainer;Kudla, Jorg
通讯作者：
Kudla, Jorg