Involvement of protein kinase C in Rickettsia rickettsii-induced transcriptional activation of the host endothelial cell.
Involvement of protein kinase C in Rickettsia rickettsii-induced transcriptional activation of the host endothelial cell.
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蛋白激酶 C 参与立克次体诱导的宿主内皮细胞转录激活。
DOI：
10.1128/iai.67.12.6418-6423.1999
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发表时间：
1999
影响因子：
3.1
通讯作者：
Sporn,LA
中科院分区：
文献类型：
--
作者：
Sahni,SK;Turpin,LC;Brown,TL;Sporn,LA
Our laboratory has reported on a biphasic pattern of nuclear factor κB (NF-κB) activation in cultured human umbilical vein endothelial cells during infection withRickettsia rickettsii, an obligate, intracellular bacterium, and the etiologic agent of Rocky Mountain spotted fever. Transcriptional activation of the tissue factor (TF) gene during this infection has been shown to involve NF-κB. To further understand the signal transduction events underlying these phenomena, we studied the role of protein kinase C (PKC), a ubiquitous family of phospholipid-dependent enzymes implicated in the regulation of a variety of cell signaling pathways. Two inhibitors of PKC, namely, bisindolylmaleimide I hydrochloride (BM-1) and calphostin C, which exhibit different inhibitory properties towards various isozymes of PKC, were used. Infection of cells withR. rickettsiiin the presence of BM-1 (50 nM) did not significantly affect NF-κB, whereas calphostin C (2.5 μM) completely blocked the early phase of NF-κB activation. The late, sustained phase also was not affected by treatment with BM-1. Downregulation of phorbol ester-sensitive PKCs by long-term treatment with phorbol 12-myristate 13-acetate (PMA) did not inhibit NF-κB activation. Likewise, this downregulation had no effect on induction of TF activity. The activity of TF was, however, sensitive to BM-1 and calphostin C, whereas expression of TF mRNA was inhibited only by calphostin C. Overall, these results suggest the lack of involvement of classical PKC pathways inR. rickettsii-induced NF-κB activation but the possible involvement of a non-PMA-responsive PKC isoform in the posttranscriptional control of TF expression.
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E-选择素依赖性中性粒细胞粘附于立克次体感染的内皮细胞。
影响因子：
20.3
作者：
Sporn,LA;Lawrence,SO;Silverman,DJ;Marder,VJ
通讯作者：
Marder,VJ
培养的人内皮细胞的立克次体感染诱导组织因子表达。
影响因子：
20.3
作者：
Sporn,LA;Haidaris,PJ;Shi,RJ;Nemerson,Y;Silverman,DJ;Marder,VJ
通讯作者：
Marder,VJ
影响因子：
4.4
作者：
S. Hobbie;Li-Mei Chen;R. Davis;J. Galán
通讯作者：
S. Hobbie;Li-Mei Chen;R. Davis;J. Galán
影响因子：
3.1
作者：
Tang, P;Sutherland, CL;Finlay, BB
通讯作者：
Finlay, BB
哺乳动物蛋白激酶 C-zeta 的过度表达不会影响 NIH 3T3 细胞的生长特性。
DOI：
--
发表时间：
1995
期刊：
Biochemical and Biophysical Research Communications - BBRC
影响因子：
--
作者：
P. Crespo;H. Mischak;J. Gutkind
通讯作者：
J. Gutkind