Non-invasive imaging of plant roots in different soils using magnetic resonance imaging (MRI).
Non-invasive imaging of plant roots in different soils using magnetic resonance imaging (MRI).
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DOI：
10.1186/s13007-017-0252-9
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发表时间：
2017
期刊：
影响因子：
5.1
通讯作者：
Koller R
中科院分区：
文献类型：
--
作者：
Pflugfelder D;Metzner R;van Dusschoten D;Reichel R;Jahnke S;Koller R
根系具有高度的可塑性，并根据其土壤环境进行适应。研究土壤对根系发育的特殊影响需要适应和评估多种基质的成像方法。可以使用磁共振成像（MRI）获得土壤中的非侵入性3D根部图像。然而，并不是所有的基质都适合MRI。使用大麦作为模型植物，我们研究了可实现的图像质量和适用性的6个市售天然土壤基质的常见土壤质地的根表型。结果进行了比较，与两个人工组成的基板先前记录的MRI根成像。在八种测试基质中的五种中，直径小于300 µm的大麦侧根仍然可以分辨。在其他两种土壤中，只有较厚的大麦种子根是可检测的。对于这两种基质，最小可检测的根直径在400和500 µm之间。只有一种土壤不允许用MRI对根部进行成像。在人工组合基质中，土壤水分超过最大持水量（WHCmax）的70%时，根系成像受阻。对于自然土壤基质，土壤水分没有影响MRI根系图像质量在50- 80%WHCmax的调查范围。几乎所有测试的天然土壤基质都允许使用MRI进行根部成像。这些基底中有一半产生的根部图像与我们目前的实验室标准基底相当，允许根部检测到300 µm的直径。这些土壤由供应商提供，特别是不需要去除铁磁性颗粒。随着不同土壤的表征，现在可以使用非侵入性MRI进行跨基质性状稳定性等调查。本文的在线版本（10.1186/s13007-017-0252-9）包含补充材料，可供授权用户使用。
Root systems are highly plastic and adapt according to their soil environment. Studying the particular influence of soils on root development necessitates the adaptation and evaluation of imaging methods for multiple substrates. Non-invasive 3D root images in soil can be obtained using magnetic resonance imaging (MRI). Not all substrates, however, are suitable for MRI. Using barley as a model plant we investigated the achievable image quality and the suitability for root phenotyping of six commercially available natural soil substrates of commonly occurring soil textures. The results are compared with two artificially composed substrates previously documented for MRI root imaging. In five out of the eight tested substrates, barley lateral roots with diameters below 300 µm could still be resolved. In two other soils, only the thicker barley seminal roots were detectable. For these two substrates the minimal detectable root diameter was between 400 and 500 µm. Only one soil did not allow imaging of the roots with MRI. In the artificially composed substrates, soil moisture above 70% of the maximal water holding capacity (WHCmax) impeded root imaging. For the natural soil substrates, soil moisture had no effect on MRI root image quality in the investigated range of 50–80% WHCmax. Almost all tested natural soil substrates allowed for root imaging using MRI. Half of these substrates resulted in root images comparable to our current lab standard substrate, allowing root detection down to a diameter of 300 µm. These soils were used as supplied by the vendor and, in particular, removal of ferromagnetic particles was not necessary. With the characterization of different soils, investigations such as trait stability across substrates are now possible using noninvasive MRI. The online version of this article (10.1186/s13007-017-0252-9) contains supplementary material, which is available to authorized users.
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通过X射线μ计算的断层扫描从土壤中恢复完整的植物根系体系。
影响因子：
5.1
作者：
Mairhofer S;Zappala S;Tracy S;Sturrock C;Bennett MJ;Mooney SJ;Pridmore TP
通讯作者：
Pridmore TP
影响因子：
6.9
作者：
Hodge, A
通讯作者：
Hodge, A
影响因子：
9.7
作者：
Cook, Freeman J.;Orchard, Valerie A.
通讯作者：
Orchard, Valerie A.
影响因子：
2.1
作者：
ROGERS, HH;BOTTOMLEY, PA
通讯作者：
BOTTOMLEY, PA
DOI：
10.1139/x91-217
发表时间：
1991-11-01
期刊：
CANADIAN JOURNAL OF FOREST RESEARCH-REVUE CANADIENNE DE RECHERCHE FORESTIERE
影响因子：
--
作者：
BROWN, DP;PRATUM, TK;PERRY, D
通讯作者：
PERRY, D