Polyunsaturated Fatty Acid-Derived Lipid Mediators as Potential Biomarkers for Leprosy Among Individuals with Asymptomatic Mycobacterium leprae Infection.
Polyunsaturated Fatty Acid-Derived Lipid Mediators as Potential Biomarkers for Leprosy Among Individuals with Asymptomatic Mycobacterium leprae Infection.
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DOI：
10.1021/acsinfecdis.2c00585
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发表时间：
2023-08-11
影响因子：
5.3
通讯作者：
Belisle JT
中科院分区：
文献类型：
--
作者：
Silva CAM;Graham BG;Webb K;Islam MN;Harton M;de Mello Marques MA;Marques de Carvalho F;Pinheiro RO;Spencer J;Sarno EN;Batista Pereira GM;Vidal Pessolani MC;Santos de Macedo C;Belisle JT
麻风患者的家庭内接触者被麻风分枝杆菌感染的风险增加，约5-10%会发展为活动性疾病。一个预后工具，以确定最大的风险，以发展为活动的疾病将加强早期麻风诊断和最佳的预防干预措施。以往的代谢组学研究表明，由ω-3和ω-6多不饱和脂肪酸(PUFA)衍生的宿主脂质介质是潜在的麻风生物标志物。在这项研究中，我们用液相色谱-质谱仪和酶联免疫分析法对麻风患者血清进行了回顾性研究，以确定与非麻风患者相比，发生麻风的患者血清中ω-3和ω-6多不饱和脂肪酸代谢产物的循环水平是否发生了变化。血清采集于首发病例诊断时及麻风临床体征/症状出现前。我们的发现表明，与HCDNL相比，HCDL血清表现出明显的代谢特征。具体来说，花生四烯酸、白三烯B4，11-羟基二十碳四烯酸、前列腺素D2和脂氧素A4在HCDL中升高。相反，前列腺素E_2水平在慢性阻塞性肺疾病中降低。ω-3多不饱和脂肪酸、二十二碳六烯酸和二十碳五烯酸以及二十二碳六烯酸衍生的解析素D1和MAREINE-1也高于非霍奇金淋巴瘤患者。PCA分析提供了进一步的证据，表明脂质介质可以作为进展为活动期麻风的早期生物标记物。LOGISTIC模型发现，在早期发现表现为麻风病的HCS方面，最有潜力的是解决素d1和d2，以及前列腺素d2。
Intra-household contacts of leprosy patients (HCs) are at increased risk of infection by Mycobacterium leprae and about ~ 5–10 % will develop active disease. A prognostic tool to identify HCs with the greatest risk of progressing to active disease would enhance early leprosy diagnosis and optimize prophylactic intervention. Previous metabolomics studies suggest that host lipid mediators derived from ω-3 and ω-6 polyunsaturated fatty acids (PUFAs) are potential biomarkers for leprosy. In this study, we investigated retrospective sera of leprosy HCs by liquid chromatography-mass spectrometry and enzyme-linked immunoassay to determine whether circulating levels of ω-3 and ω-6 PUFA metabolites were altered in HCs that developed leprosy (HCDLs) in comparison to those that did not (HCNDLs). Sera were collected from HCs at the time of index case diagnosis and before clinical signs/symptoms of leprosy. Our findings showed that HCDL sera exhibited a distinct metabolic profile in comparison to HCDNL. Specifically, arachidonic acid, leukotriene B4,11-hydroxyeicosatetraenoic acid, prostaglandin D2 and lipoxin A4 were elevated in HCDL. In contrast, prostaglandin E2 levels were reduced in HCDL. The ω-3 PUFAs, docosahexaenoic acid and eicosapentaenoic acid, and the docosahexaenoic acid-derived resolvin D1 and maresin-1 were also elevated in HCDL individuals compared to HCNDL. PCA analyses provided further evidence that lipid mediators could serve as an early biomarker for progression to active leprosy. A logistics model identified resolvin D1 and D2, and prostaglandin D2 as having the greatest potential for early detection of HCs that will manifest leprosy.
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影响因子：
3.5
作者：
Casado-Diaz, Antonio;Ferreiro-Vera, Carlos;Manuel Quesada-Gomez, Jose
通讯作者：
Manuel Quesada-Gomez, Jose
DOI：
10.1111/j.2517-6161.1995.tb02031.x
发表时间：
1995-01-01
影响因子：
5.8
作者：
BENJAMINI, Y;HOCHBERG, Y
通讯作者：
HOCHBERG, Y
影响因子：
9.5
作者：
Aittokallio, Tero;Schwikowski, Benno
通讯作者：
Schwikowski, Benno
影响因子：
14.9
作者：
Pang Z;Chong J;Zhou G;de Lima Morais DA;Chang L;Barrette M;Gauthier C;Jacques PÉ;Li S;Xia J
通讯作者：
Xia J
影响因子：
3.8
作者：
da Silva MB;Portela JM;Li W;Jackson M;Gonzalez-Juarrero M;Hidalgo AS;Belisle JT;Bouth RC;Gobbo AR;Barreto JG;Minervino AHH;Cole ST;Avanzi C;Busso P;Frade MAC;Geluk A;Salgado CG;Spencer JS
通讯作者：
Spencer JS