Neuropeptide tyrosine in the rat adrenal gland—immunohistochemical and in situ hybridization studies
Neuropeptide tyrosine in the rat adrenal gland—immunohistochemical and in situ hybridization studies
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大鼠肾上腺中的神经肽酪氨酸——免疫组织化学和原位杂交研究
DOI：
10.1016/0306-4522(88)90335-1
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发表时间：
1988
期刊：
影响因子：
3.3
通讯作者：
M. Goldstein
中科院分区：
文献类型：
--
作者：
M. Schalling;K. Seroogy;T. Hökfelt;S. Chai;H. Hallman;H. Persson;D. Larhammar;A. Ericsson;L. Terenius;J. Graffi;J. Massoulié;M. Goldstein
使用免疫组织化学和针对神经肽酪氨酸、儿茶酚胺合成酶酪氨酸羟化酶、苯乙醇胺-N-甲基转移酶和乙酰胆碱酯酶的抗血清来分析大鼠的肾上腺，并使用编码大鼠神经肽外显子2的切口翻译的280碱基对脱氧核糖核酸探针进行原位杂交。酪氨酸基因。在三种结构中观察到神经肽酪氨酸样免疫反应性：嗜铬细胞、髓质神经节细胞和神经纤维。嗜铬细胞为去甲肾上腺素型和肾上腺素型。神经节细胞似乎不含有任何儿茶酚胺合成酶，但对乙酰胆碱酯酶表现出强烈的免疫反应。因此，它们很可能是胆碱能神经元。 使用切口翻译的脱氧核糖核酸探针与大鼠神经肽酪氨酸信使核糖核酸进行原位杂交，显示神经节细胞上有非常高的颗粒密度，嗜铬细胞上有中等密度，皮质上有低背景，这与神经肽酪氨酸样免疫反应性的免疫组织化学证明一致。嗜铬细胞和神经节细胞。强烈的神经肽酪氨酸样免疫反应性和神经肽酪氨酸信使核糖核酸的低含量表明嗜铬细胞具有相当大的肽储存，但肽周转率较低。相反，神经节细胞体似乎含有少量的神经肽酪氨酸样免疫反应性，但表现出较高的神经肽酪氨酸合成率。对胺消耗药物利血平的初步研究表明，神经节细胞和髓质细胞中的信使核糖核酸均有所增加。在嗜铬细胞中，注射后 3 天和 4 天出现最高活性，8 天后水平降至正常。目前的研究结果证明了肾上腺髓质中两个细胞群中神经肽酪氨酸的合成和储存。原位杂交及其细胞分辨率可以提供有关药物和实验程序对这两种神经肽酪氨酸储存可能产生的差异效应的信息。
The adrenal gland of the rat was analysed with immunohistochemistry and antisera to neuropeptide tyrosine, to the catecholamine-synthesizing enzymes tyrosine hydroxylase,phenylethanolamine-N-methyltransferase, and to acetylcholinesterase and within situhybridization using a nick-translated 280 base pair deoxyribonucleic acid probe coding for exon 2 of the rat neuropeptide tyrosine gene. Neuropeptide tyrosine-like immunoreactivity was observed in three structures: chromaffin cells, medullary ganglion cells and nerve fibers. The chromaffin cells were of both the noradrenaline- and adrenaline-type. The ganglion cells did not seem to contain any catecholamine-synthesizing enzymes but exhibited a strong immunoreaction for acetylcholinesterase. They were thus in all probability cholinergic neurons.In situhybridization using the nick-translated deoxyribonucleic acid probe to rat neuropeptide tyrosine messenger ribonucleic acid revealed a very high-grain density over the ganglion cells, a moderate density over the chromaffin cells and a low background over cortex, in agreement with the immunohistochemical demonstration of neuropeptide tyrosine-like immunoreactivity both in chromaffin and ganglion cells. The intense neuropeptide tyrosine-like immunoreactivity and low content of neuropeptide tyrosine messenger ribonucleic acid suggest that the chromaffin cells have fairly large peptide stores but that the peptide turnover is low. In contrast, the ganglion cell bodies seem to contain low amounts of neuropeptide tyrosine-like immunoreactivity but exhibit a high neuropeptide tyrosine synthesis rate. Preliminary studies with the amine-depleting drug reserpine revealed an increase in messenger ribonucleic acid both in ganglion cells and medullary cells. In the chromaffin cells the highest activity was seen 3 and 4 days after injection, and the levels were down to normal after 8 days.The present findings demonstrate neuropeptide tyrosine synthesis and storage in two cell populations in the adrenal medulla.In situhybridization with its cellular resolution can provide information on possible differential effects of drugs and experimental procedures on these two neuropeptide tyrosine stores.
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DOI：
10.1089/dna.1983.2.157
发表时间：
1983
期刊：
DNA (Mary Ann Liebert, Inc.)
影响因子：
--
作者：
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DOI：
--
发表时间：
1986
期刊：
The Journal of biological chemistry
影响因子：
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通讯作者：
Dixon,JE
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DOI：
10.1177/34.1.3510246
发表时间：
1986
期刊：
The journal of histochemistry and cytochemistry : official journal of the Histochemistry Society
影响因子：
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作者：
Shivers,BD;Harlan,RE;Pfaff,DW;Schachter,BS
通讯作者：
Schachter,BS
DOI：
10.1073/pnas.81.14.4577
发表时间：
1984-01-01
期刊：
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA-BIOLOGICAL SCIENCES
影响因子：
--
作者：
MINTH, CD;BLOOM, SR;DIXON, JE
通讯作者：
DIXON, JE
对苯二胺可延缓免疫荧光的褪色并增强其强度。
DOI：
10.1177/31.6.6341464
发表时间：
1983
期刊：
The journal of histochemistry and cytochemistry : official journal of the Histochemistry Society
影响因子：
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作者：
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通讯作者：
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