Opposing effects of MYZUS PERSICAE-INDUCED LIPASE 1 and jasmonic acid influence the outcome of Arabidopsis thaliana-Fusarium graminearum interaction.
Opposing effects of MYZUS PERSICAE-INDUCED LIPASE 1 and jasmonic acid influence the outcome of Arabidopsis thaliana-Fusarium graminearum interaction.
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DOI：
10.1111/mpp.13216
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发表时间：
2022-08
影响因子：
4.9
通讯作者：
Shah, Jyoti
中科院分区：
文献类型：
--
作者：
Alam, Syeda T.;Sarowar, Sujon;Mondal, Hossain A.;Makandar, Ragiba;Chowdhury, Zulkarnain;Louis, Joe;Shah, Jyoti
禾谷镰刀菌（Fg）是小颗粒谷物的重要真菌病原菌，也能感染拟南芥。在拟南芥中，茉莉酸（JA）信号传导涉及JASMONATE RESISTANT 1（JAR 1），其合成JA-异亮氨酸，JA的信号传导形式，促进对Fg的易感性。在这里，我们表明，拟南芥MYZUS PERSICAE诱导的脂肪酶1（MPL 1），通过其限制JA积累的影响，限制Fg感染。MPL 1的表达在Fg感染时上调，而过表达MPL 1的MPL 1 ‐OE植物表现出对Fg的抗性增强。相比之下，mpl 1突变体的疾病严重程度高于野生型。JA含量在MPL 1-OE中较低，而在mpl 1中高于野生型，表明MPL 1限制了JA积累。药理学实验证实了由MPL 1决定的限制JA积累对减少Fg感染的重要性。甲基-JA应用减弱了MPL 1-OE-赋予的抗性，而JA生物合成抑制剂布洛芬增强了mpl 1中的抗性。此外，JA生物合成缺陷型opr 3突变体对mpll具有上位性，导致mpll opr 3植物中的抗性增强。相比之下，JAR 1对于mpl 1赋予的对Fg的易感性不是必需的。考虑到甲基JA促进培养物中的Fg生长，我们认为MPL 1通过限制植物来源的Fg生长促进因子的可用性来部分减少疾病。 MYZUS PERSICAE诱导的脂肪酶1（MPL 1）通过限制茉莉酸的积累来减少拟南芥中禾谷镰刀菌的感染。
Fusarium graminearum (Fg) is an important fungal pathogen of small grain cereals that can also infect Arabidopsis thaliana. In Arabidopsis, jasmonic acid (JA) signalling involving JASMONATE RESISTANT 1 (JAR1), which synthesizes JA‐isoleucine, a signalling form of JA, promotes susceptibility to Fg. Here we show that Arabidopsis MYZUS PERSICAE‐INDUCED LIPASE 1 (MPL1), via its influence on limiting JA accumulation, restricts Fg infection. MPL1 expression was up‐regulated in response to Fg infection, and MPL1‐OE plants, which overexpress MPL1, exhibited enhanced resistance against Fg. In comparison, disease severity was higher on the mpl1 mutant than the wild type. JA content was lower in MPL1‐OE and higher in mpl1 than in the wild type, indicating that MPL1 limits JA accumulation. Pharmacological experiments confirmed the importance of MPL1‐determined restriction of JA accumulation on curtailment of Fg infection. Methyl‐JA application attenuated the MPL1‐OE‐conferred resistance, while the JA biosynthesis inhibitor ibuprofen enhanced resistance in mpl1. Also, the JA biosynthesis‐defective opr3 mutant was epistatic to mpl1, resulting in enhanced resistance in mpl1 opr3 plants. In comparison, JAR1 was not essential for the mpl1‐conferred susceptibility to Fg. Considering that methyl‐JA promotes Fg growth in culture, we suggest that in part MPL1 curtails disease by limiting the availability of a plant‐derived Fg growth‐promoting factor. MYZUS PERSICAE‐INDUCED LIPASE 1 (MPL1) curtails Fusarium graminearum infection in Arabidopsis thaliana by limiting jasmonic acid accumulation.
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