Structural principles that enable oligomeric small heat-shock protein paralogs to evolve distinct functions.
Structural principles that enable oligomeric small heat-shock protein paralogs to evolve distinct functions.
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DOI：
10.1126/science.aam7229
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发表时间：
2018-02-23
期刊：
影响因子：
--
通讯作者：
Benesch JLP
中科院分区：
文献类型：
--
作者：
Hochberg GKA;Shepherd DA;Marklund EG;Santhanagoplan I;Degiacomi MT;Laganowsky A;Allison TM;Basha E;Marty MT;Galpin MR;Struwe WB;Baldwin AJ;Vierling E;Benesch JLP
寡聚体蛋白质以显著的选择性组装，即使在密切相关的蛋白质存在下，以执行其细胞作用。我们发现，大多数蛋白质相关的基因重复的寡聚祖先已经进化到避免异源寡聚化，这与他们的收购不同的功能。我们报告如何避免两个低聚的小热休克蛋白旁系同源物的共组装。组装的层次结构，包括在平衡时只短暂填充的中间体，确保了选择性低聚。单体中非界面区域的构象灵活性阻止了共组装，使界面在很大程度上保持保守。同聚寡聚体必须克服共组装的熵益处，因此，同聚旁系同源物包含比不具有旁系同源物的同聚物更少的亚基。小的热休克蛋白避免功能失调的共组装使用的机制，造成最小的破坏，其保守的接口
Oligomeric proteins assemble with remarkable selectivity, even in the presence of closely related proteins, in order to perform their cellular roles. We show that most proteins related by gene duplication of an oligomeric ancestor have evolved to avoid hetero-oligomerization, and that this correlates with their acquisition of distinct functions. We report how co-assembly is avoided by two oligomeric small heat-shock protein paralogs. A hierarchy of assembly, involving intermediates that are populated only fleetingly at equilibrium, ensures selective oligomerisation. Conformational flexibility at non-interfacial regions in the monomers prevents co-assembly, allowing interfaces to remain largely conserved. Homomeric oligomers must overcome the entropic benefit of co-assembly and, accordingly, homomeric paralogs comprise fewer subunits than homomers that have no paralogs. Small heat-shock proteins avoid dysfunctional co-assembly using mechanisms that cause minimal disruption to their conserved interfaces
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影响因子：
4.8
作者：
Basha, Eman;Jones, Christopher;Vierling, Elizabeth
通讯作者：
Vierling, Elizabeth
影响因子：
7.4
作者：
Helm, KW;Lee, GJ;Vierling, E
通讯作者：
Vierling, E
通过混杂中间体发展出新的蛋白质 - 蛋白质相互作用特异性。
影响因子：
64.5
作者：
Aakre CD;Herrou J;Phung TN;Perchuk BS;Crosson S;Laub MT
通讯作者：
Laub MT
DOI：
10.1126/science.1240810
发表时间：
2013-10-04
期刊：
Science (New York, N.Y.)
影响因子：
--
作者：
Baker CR;Hanson-Smith V;Johnson AD
通讯作者：
Johnson AD
影响因子：
56.9
作者：
Perica, Tina;Kondo, Yasushi;Teichmann, Sarah A.
通讯作者：
Teichmann, Sarah A.