A comparative study of the Arabidopsis thaliana guard-cell transcriptome and its modulation by sucrose.
A comparative study of the Arabidopsis thaliana guard-cell transcriptome and its modulation by sucrose.
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DOI：
10.1371/journal.pone.0049641
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发表时间：
2012
期刊：
影响因子：
3.7
通讯作者：
Jones AM
中科院分区：
文献类型：
--
作者：
Bates GW;Rosenthal DM;Sun J;Chattopadhyay M;Peffer E;Yang J;Ort DR;Jones AM
对从拟南芥叶片中人工切下的保卫细胞分离的RNA进行微阵列分析。通过将我们的数据与拟南芥保卫细胞原生质体的两项早期研究相结合，我们提供了保卫细胞转录组的强大视图，该转录组富含转录因子，信号蛋白，转运蛋白和碳水化合物修饰酶的转录物。为了验证光合作用产生的糖信号保卫细胞调节气孔开放的假设，我们确定了已用蔗糖处理的叶片保卫细胞中表达的基因的概况。结果显示，保卫细胞中440个基因的表达因蔗糖而发生变化。与这一假设相一致，这些基因编码光合作用和糖，水，氨基酸和离子运输的细胞功能。植物的T-DNA插入系的50个基因高度响应蔗糖进行了检查，在保卫细胞功能的缺陷。12个以前不知道在保卫细胞功能的基因被证明是重要的叶电导，水分利用效率，和/或气孔发育。其中，有三个是特别感兴趣的，几乎在每一个气孔功能测试中显示出效果，而不改变气孔密度：TPS 5（At 4g 17770），TRAF结构域含有蛋白（At 1g 65370），和WD重复含有蛋白（At 1g 15440）。
Microarray analysis was performed on RNA isolated from guard cells that were manually dissected from leaves of Arabidopsis. By pooling our data with those of two earlier studies on Arabidopsis guard cell protoplasts, we provide a robust view of the guard-cell transcriptome, which is rich in transcripts for transcription factors, signaling proteins, transporters, and carbohydrate-modifying enzymes. To test the hypothesis that photosynthesis-derived sugar signals guard cells to adjust stomatal opening, we determined the profile of genes expressed in guard cells from leaves that had been treated with sucrose. The results revealed that expression of 440 genes changed in guard cells in response to sucrose. Consistent with this hypothesis, these genes encoded cellular functions for photosynthesis and transport of sugars, water, amino acids, and ions. Plants of T-DNA insertion lines for 50 genes highly responsive to sucrose were examined for defects in guard cell function. Twelve genes not previously known to function in guard cells were shown to be important in leaf conductance, water-use efficiency, and/or stomate development. Of these, three are of particular interest, having shown effects in nearly every test of stomatal function without a change in stomatal density: TPS5 (At4g17770), a TRAF domain-containing protein (At1g65370), and a WD repeat–containing protein (At1g15440).
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DOI：
10.1073/pnas.0907205106
发表时间：
2009-12-01
影响因子：
11.1
作者：
Jammes, Fabien;Song, Charlotte;Kwak, June M.
通讯作者：
Kwak, June M.
影响因子：
12.3
作者：
Gentleman RC;Carey VJ;Bates DM;Bolstad B;Dettling M;Dudoit S;Ellis B;Gautier L;Ge Y;Gentry J;Hornik K;Hothorn T;Huber W;Iacus S;Irizarry R;Leisch F;Li C;Maechler M;Rossini AJ;Sawitzki G;Smith C;Smyth G;Tierney L;Yang JY;Zhang J
通讯作者：
Zhang J
影响因子：
11.6
作者：
Leonhardt, N;Kwak, JM;Schroeder, JI
通讯作者：
Schroeder, JI
影响因子：
3.5
作者：
Grigston, Jeffery C.;Osuna, Daniel;Scheible, Wolf-Ruediger;Liu, Chenggang;Stitt, Mark;Jones, Alan M.
通讯作者：
Jones, Alan M.
影响因子：
6.9
作者：
Kang, Yun;Outlaw, William H., Jr.;Riddle, Kimberly A.
通讯作者：
Riddle, Kimberly A.