IN VITRO EFFECTS OF HYPOTHALAMIC–PITUITARY–ADRENAL AXIS (HPA) HORMONES ON SCHISTOSOMA MANSONI
IN VITRO EFFECTS OF HYPOTHALAMIC–PITUITARY–ADRENAL AXIS (HPA) HORMONES ON SCHISTOSOMA MANSONI
复制标题
下丘脑-垂体-肾上腺轴 (HPA) 激素对曼索尼血吸虫的体外影响
作者：
J. Morales;Fawzi Mohamed;A. Ghaleb;S. Baig;C. Hallal;R. Damian
本文报道了4种下丘脑-垂体-肾上腺（HPA）轴激素对曼氏血吸虫尾蚴、童虫和蠕虫的体外处理效果。脱氢表雄酮（DHEA）对活力的影响最强。尾蚴比童虫和成虫对该激素更敏感。机械转化的青少年表现出100%的死亡率（显微镜下测定进行性内部解体，在细胞质中的透亮区的发展，和运动性的逐步丧失）48小时后，而生理诱导的青少年更耐，保持活力长达5天的曝光。雄鼠对DHEA的致死作用的敏感性明显低于雌鼠。当蠕虫配对时，DHEA致死率显著降低，仅在培养4天后活力开始下降。皮质醇降低了每个测试阶段的生存能力。促肾上腺皮质激素释放激素（CRH）和促肾上腺皮质激素（ACTH）不影响任何阶段的生存能力。DHEA和皮质醇显着抑制体外产卵，而CRH和ACTH没有。脱氢表雄酮和皮质醇以浓度依赖的方式影响雌虫的存活和产卵。这些结果为控制血吸虫病提供了可能的新途径。
The effects of in vitro treatment of cercariae, schistosomula, and adult worms of Schistosoma mansoni with 4 hypothalamic–pituitary–adrenal (HPA) axis hormones are described. Dehydroepiandrosterone (DHEA) had the strongest effect on viability. Cercariae were more susceptible to this hormone than schistosomula and adults. Mechanically transformed schistosomula showed 100% mortality (determined microscopically by progressive internal disorganization, development of lucent areas in the cytoplasm, and progressive loss of motility) after 48 hr, whereas physiologically induced schistosomula were more resistant, maintaining viability for up to 5 days of exposure. Males were considerably less sensitive than females to the lethal action of DHEA. When adult worms were paired, DHEA lethality was markedly reduced, with viability beginning to decrease only after 4 days in culture. Cortisol reduced the viability of each of the stages tested about equally. Corticotropin-releasing hormone (CRH) and adrenocorticotropin (ACTH) did not affect the viability of any stage. DHEA and cortisol significantly inhibited in vitro oviposition, whereas CRH and ACTH did not. DHEA and cortisol exerted their effects on schistosome viability and oviposition in a concentration-dependent manner. These results suggest possible new avenues for the control of schistosomiasis.
影响因子：
2.1
作者：
M. Nakazawa;M. Fantappíé;G. Freeman;S. Elói-Santos;N. Olsen;W. Kovacs;W. Secor;D. Colley
通讯作者：
M. Nakazawa;M. Fantappíé;G. Freeman;S. Elói-Santos;N. Olsen;W. Kovacs;W. Secor;D. Colley
影响因子：
2.1
作者：
ELOISANTOS, S;OLSEN, NJ;COLLEY, DG
通讯作者：
COLLEY, DG