Matrix-free detection of intact ions from proteins in argon-cluster secondary ion mass spectrometry.
Matrix-free detection of intact ions from proteins in argon-cluster secondary ion mass spectrometry.
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在氩簇二次离子质谱中对蛋白质的完整离子进行无基质检测。
DOI：
10.1002/rcm.3922
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发表时间：
2009
期刊：
影响因子：
--
通讯作者：
N. Toyoda
中科院分区：
文献类型：
--
作者：
K. Mochiji;M. Hashinokuchi;K. Moritani;N. Toyoda
在有机物质的二次离子质谱法（SIMS）中，目前可检测到的完整离子的分子量最多只有1000 Da，这使得该技术无法应用于更高质量的生物材料。我们已经开发了SIMS仪器，其中主要离子是氩簇离子，每个原子的动能被控制在1ev以下。将该仪器应用于多种多肽和蛋白质，当每个原子的动能低于5 eV时，片段离子的信号强度降低了10(2)倍；此外，在不使用任何基质的情况下，检测胰岛素（分子量为5808）和细胞色素C（分子量为12 327）的完整离子。这些结果表明，当每个原子的动能降低到目标的解离能水平时，可以在不牺牲完整离子溅射产率的情况下有效地抑制碎片化。这一原理完全适用于其他生物分子，有望为SIMS在未来生物材料中的应用做出贡献。
In the secondary ion mass spectrometry (SIMS) of organic substances, the molecular weight of the intact ions currently detectable is at best only as high as 1000 Da, which for all practical purposes prevents the technique from being applied to biomaterials of higher mass. We have developed SIMS instrumentation in which the primary ions were argon cluster ions having a kinetic energy per atom, controlled down to 1 eV. On applying this instrumentation to several peptides and proteins, the signal intensity of fragment ions was decreased by a factor of 10(2) when the kinetic energy per atom was decreased below 5 eV; moreover, intact ions of insulin (molecular weight (MW): 5808) and cytochrome C (MW: 12 327) were detected without using any matrix. These results indicate that fragmentation can be substantially suppressed without sacrificing the sputter yield of intact ions when the kinetic energy per atom is decreased to the level of the target's dissociation energy. This principle is fully applicable to other biomolecules, and it can thus be expected to contribute to applications of SIMS to biomaterials in the future.
DOI：
10.1002/rcm.1290081218
发表时间：
1994-12
期刊：
Rapid communications in mass spectrometry : RCM
影响因子：
--
作者：
D. Cornett;Terry D. Lee;J. Mahoney;P. Todd
通讯作者：
D. Cornett;Terry D. Lee;J. Mahoney;P. Todd