Micropatterned photodegradable hydrogels for the sorting of microbeads and cells.
Micropatterned photodegradable hydrogels for the sorting of microbeads and cells.
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DOI：
10.1002/anie.201303965
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发表时间：
2013-08-26
影响因子：
16.6
通讯作者：
Revzin, Alexander
中科院分区：
文献类型：
--
作者：
Siltanen, Christian;Shin, Dong-Sik;Sutcliffe, Julie;Revzin, Alexander
生物分子和细胞的微阵列在基因组学、诊断和药物筛选中发挥着重要作用微图案表面上的活细胞捕获对于诊断或生物传感应用特别有价值，因为细胞阵列可以快速表征和分选。例如，抗体包被微柱阵列已被用于高保真地检测和分离外周血中罕见的循环肿瘤细胞微孔阵列已被用于捕获和分析单个免疫细胞虽然从这些阵列中释放或分类细胞亚群以进行更彻底的下游分析（例如通过PCR或Western blot）通常很重要，但从微图案表面检索特定细胞的方法仍然有限。电化学刺激可以利用微模式内的特定区域释放细胞，b[5]但这些方法需要用电极注册细胞阵列，并且它们可以成为**作者感谢Sven Hausner和Kevin Lau在实验设计方面的帮助。本工作由NIH资助（C4EB012836-01）给JS和NSF EFRI资助（0937997）给AR©2013 Wiley-VCH Verlag GmbH & Co. KGaA， Weinheim通讯：dongsik Shin， dshin@ ucdavis。edu;Alexander Revzin, arevzin@ ucdavis。edu。本文的支持信息可以在WWW的http://dx下找到。doi。org/10.1002/anie。201303965.
Microarrays of biomolecules and cells have played important roles in genomics, diagnostics, and drug screening.[1] Live-cell capture on micropatterned surfaces is particularly valuable for diagnostic or biosensing applications,[2] as cell arrays are amenable to rapid characterization and sorting. For example, antibody-coated micropost arrays have been used to detect and isolate rare circulating tumor cells from peripheral blood with high fidelity.[3] Microwell arrays have been used for the capture and analysis of single immune cells.[4] While it is often important to release or sort subsets of cells from these arrays for more thorough downstream analysis (eg by PCR or Western blot), methods for the retrieval of specific cells from micropatterned surfaces remain limited. Electrochemical stimulation can be exploited to release cells from particular regions within micropatterns,[5] but these approaches require that cell arrays be registered with electrodes, and they can become** The authors gratefully acknowledge Sven Hausner and Kevin Lau for their assistance with experimental design. This work was supported by an NIH grant (C4EB012836-01) to JS and an NSF EFRI grant (0937997) to AR© 2013 Wiley-VCH Verlag GmbH & Co. KGaA, Weinheim Correspondence to: Dong-Sik Shin, dshin@ ucdavis. edu; Alexander Revzin, arevzin@ ucdavis. edu. Supporting information for this article is available on the WWW under http://dx. doi. org/10.1002/anie. 201303965.
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