Determination of optimal rhodamine fluorophore for in vivo optical imaging.
Determination of optimal rhodamine fluorophore for in vivo optical imaging.
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DOI：
10.1021/bc800140c
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发表时间：
2008-08
影响因子：
4.7
通讯作者：
Kobayashi, Hisataka
中科院分区：
文献类型：
--
作者：
Longmire, Michelle R.;Ogawa, Mikako;Hama, Yukihiro;Kosaka, Nobuyuki;Regino, Celeste A. S.;Choyke, Peter L.;Kobayashi, Hisataka
光学成像有可能提高手术和内窥镜方法治疗癌症的疗效;然而，最佳类型的荧光探针尚未建立。众所周知，罗丹明核衍生的荧光团提供了所需性质的组合，例如良好的光稳定性、高消光系数和高荧光量子产率。然而，尽管普遍使用的罗丹明荧光团在体内光学成像，它仍然有待确定，但是，如果独特的化学性质之间的个别罗丹明核心家族成员影响荧光团参数的关键在体内光学成像应用。这些参数包括：在低pH环境中保持荧光强度，类似于内溶酶体的荧光强度;尽管靶蛋白的构象变化可能发生在苛刻的亚细胞环境中，但仍有有效的荧光信号;细胞内化后荧光的持久性;以及足够的信号背景比，以允许鉴定荧光团靶向的肿瘤。在本研究中，我们将4种常见的基于罗丹明核心的荧光染料缀合到临床上可行且快速内化的D-半乳糖受体靶向试剂半乳糖胺血清白蛋白（GmSA）上，并使用转移性卵巢癌小鼠模型进行了一系列体外和体内实验，以确定罗丹明荧光团之间是否存在差异，如果存在，该罗丹明核具有用于体内成像应用的最佳特性。在这里，我们证明了罗丹明荧光团，塔姆拉，是最强大的4个常见的罗丹明荧光团在体内光学成像卵巢癌转移到腹膜。
Optical imaging has the potential to improve the efficacy of surgical and endoscopic approaches to cancer treatment; however, the optimal type of fluorescent probe has not yet been established. It is well known that rhodamine-core-derived fluorophores offer a combination of desirable properties such as good photostability, high extinction coefficient, and high fluorescence quantum yield. However, despite the ubiquitous use of rhodamine fluorophores for in vivo optical imaging, it remains to be determined, however, if unique chemical properties among individual rhodamine core family members affect fluorophore parameters critical to in vivo optical imaging applications. These parameters include: preserved fluorescence intensity in low pH environments, similar to that of the endolysosome; efficient fluorescence signal despite conformational changes to targeting proteins as may occur in harsh subcellular environments; persistence of fluorescence after cellular internalization; and sufficient signal-to-background ratios to permit the identification of fluorophore-targeted tumors. In the present study, we conjugated 4 common rhodamine-core based fluorescent dyes to a clinically feasible and quickly internalizing D-galactose receptor targeting reagent, galactosamine serum albumin (GmSA), and conducted a series of in vitro and in vivo experiments using a metastatic ovarian cancer mouse model to determine if differences exist among rhodamine fluorophores and if so, which rhodamine core possesses optimal characteristics for in vivo imaging applications. Herein, we demonstrate that the rhodamine-fluorophore, TAMRA, is the most robust of the 4 common rhodamine fluorophores for in vivo optical imaging of ovarian cancer metastases to the peritoneum.
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影响因子：
15
作者：
Urano, Y;Kamiya, M;Nagano, T
通讯作者：
Nagano, T
影响因子：
17.3
作者：
Hoffman, Robert M.
通讯作者：
Hoffman, Robert M.
影响因子：
11.5
作者：
Hama, Yukihiro;Urano, Yasuteru;Kobayashi, Hisataka
通讯作者：
Kobayashi, Hisataka
影响因子：
4.7
作者：
Hama, Yukihiro;Urano, Yasuteru;Kobayashi, Hisataka
通讯作者：
Kobayashi, Hisataka
DOI：
10.3390/s8021157
发表时间：
2008-02-22
期刊：
Sensors (Basel, Switzerland)
影响因子：
--
作者：
Hoffman RM
通讯作者：
Hoffman RM