Keratan sulfate glycosaminoglycan and the association with collagen fibrils in rudimentary lamellae in the developing avian cornea.
Keratan sulfate glycosaminoglycan and the association with collagen fibrils in rudimentary lamellae in the developing avian cornea.
复制标题
硫酸角质素糖胺聚糖及其与发育中的禽类角膜的基本片层中的胶原纤维的关联。
DOI：
--
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发表时间：
2007
影响因子：
4.4
通讯作者：
A. Quantock
中科院分区：
文献类型：
--
作者：
Robert D. Young;E. Gealy;M. Liles;B. Caterson;J. Ralphs;A. Quantock
目的
硫酸角蛋白(KS)作为KS取代的蛋白多糖(KS PG)与胶原纤维结合，被认为在角膜基质的结构发育中起重要作用。作者使用了两种不同的硫酸盐基序特异性抗体来鉴定在鸟类角膜形态发生过程中硫酸盐KS的出现顺序及其与胶原的关系。
方法
用5D4和1B4的单抗分别与KS上的高硫酸盐化和低硫酸盐化的表位反应，用免疫荧光和免疫电子显微镜观察了鸡胚从孵化第8天到第18天的整个发育过程中的角膜。
结果
KS在孵化第8天被鉴定为点状标记，这是被检查的最早阶段，表明KS是与细胞相关的分布。到第10天，标记更加均匀，表明KS硫化基序存在于基质细胞外基质中。从第12天到第14天，免疫阳性部位主要集中在前基质中，但到第18天时，免疫阳性部位在整个基质厚度中变得更加均匀。从第10天开始，电子显微镜显示高硫酸盐化的KS表位与排列规则的胶原纤维束密切相关，最初位于发育不全的板层的细胞表面附近。单个细胞与具有不同纤维取向的胶原束相关，暗示着同时沉积多个板层的可能性。
结论
在鸡角膜形态发生过程中，高硫化KS表位的基质沉积在第10天时发生，随后以从前到后的方式积累。高硫化的KS可能有助于确定胶原纤维在新挤出到细胞外环境中的束中的规则空间组织。
PURPOSE
Keratan sulfate (KS), through its association with fibrillar collagen as KS-substituted proteoglycan (KS PG), is thought to be instrumental in the structural development of the corneal stroma. The authors used two different sulfate motif-specific antibodies to identify the sequence of appearance, and the association with collagen, of sulfated KS during avian corneal morphogenesis.
METHODS
Corneas from chicken embryos throughout the developmental period, from day 8 through day 18 of incubation, were examined by immunofluorescence and immunoelectron microscopy using monoclonal antibodies 5D4 and 1B4, which react with high- and low-sulfated epitopes on KS, respectively.
RESULTS
KS was identified as punctate labeling at incubation day 8, the earliest stage examined, suggesting a cell-associated distribution. By day 10, labeling was more homogeneous, indicating that KS sulfation motifs were present in the stromal extracellular matrix. At day 12 through day 14, immunopositive sites were concentrated primarily in the anterior stroma but became more uniform throughout the full stromal thickness by day 18. From day 10 on, electron microscopy revealed a high-sulfated KS epitope closely associated with bundles of regularly arranged collagen fibrils, initially near cell surfaces in rudimentary lamellae. Individual cells, associated with collagen bundles with different fibril orientations, imply the potential for simultaneous deposition of multiple lamellae.
CONCLUSIONS
During chick corneal morphogenesis, significant matrix deposition of high-sulfated KS epitope occurs by day 10, with accumulation subsequently proceeding in an anterior-to-posterior manner. High-sulfated KS likely serves to help define the regular spatial organization of collagen fibrils in bundles newly extruded into the extracellular milieu.
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影响因子：
17.8
作者：
Linsenmayer,TF;Fitch,JM;Gordon,MK;Cai,CX;Igoe,F;Marchant,JK;Birk,DE
通讯作者：
Birk,DE
影响因子：
4.4
作者：
Yuntao Zhang;A. H. Conrad;E. Tasheva;K. An;L. Corpuz;Y. Kariya;Kiyoshi Suzuki;G. Conrad
通讯作者：
Yuntao Zhang;A. H. Conrad;E. Tasheva;K. An;L. Corpuz;Y. Kariya;Kiyoshi Suzuki;G. Conrad
针对结缔组织蛋白聚糖的单克隆抗体的生产和表征。
DOI：
--
发表时间：
1985
期刊：
Federation proceedings
影响因子：
--
作者：
Caterson,B;Christner,JE;Baker,JR;Couchman,JR
通讯作者：
Couchman,JR
三种硫酸角质素蛋白多糖亚型的独特糖基化。
DOI：
--
发表时间：
1991
期刊：
The Journal of biological chemistry
影响因子：
--
作者：
Funderburgh,JL;Funderburgh,ML;Mann,MM;Conrad,GW
通讯作者：
Conrad,GW