Intestinal organoid culture model is a valuable system to study epithelial barrier function in IBD
Intestinal organoid culture model is a valuable system to study epithelial barrier function in IBD
复制标题
肠类器官培养模型是研究 IBD 上皮屏障功能的有价值的系统
作者：
P. Xu;H. Becker;M. Elizalde;A. Masclee;D. Jonkers
我们怀着极大的兴趣阅读了Dotti等人的文章，1他们分析了从UC患者和非IBD对照产生的上皮类器官培养物（EpOC）的转录特征。他们确定了一组在UC和对照EpOCs之间差异表达的基因，其中几个与上皮分泌和抗菌防御相关。作者进一步指出，类器官培养系统可作为探索IBD患者肠上皮的模型。因此，我们提供了新的支持，肠类器官培养也是一个有价值的模型，以研究上皮屏障功能，这已成为IBD发病机制的标志。
虽然在IBD患者的活检中研究了与上皮连接复合物相关的基因和蛋白质的表达，但肠屏障的功能分析仍然具有挑战性。为此，我们建立了一个EpOCs库，从...
With great interest, we have read the article by Dotti et al ,1 who analysed the transcriptional signature of epithelial organoid cultures (EpOCs) generated from patients with UC and non-IBD controls. They identified a set of genes that were differentially expressed between UC and control EpOCs, among which several were associated with epithelial secretory and antimicrobial defense. The authors further stated that the organoid culture system could serve as a model to explore the intestinal epithelium of patients with IBD. We hereby provide new support that intestinal organoid culture is also a valuable model to study epithelial barrier function, which has emerged as hallmark for IBD pathogenesis.
Although expression of genes and proteins related to epithelial junctional complex has been investigated in biopsies of patients with IBD, functional analyses of the intestinal barrier remains challenging. To this end, we established an EpOCs library from …
影响因子：
3.4
作者：
Capaldo, Christopher T.;Nusrat, Asma
通讯作者：
Nusrat, Asma