Investigating the resistance levels and mechanisms to penoxsulam and cyhalofop-butyl in barnyardgrass (Echinochloa crus-galli) from Ningxia Province, China
Investigating the resistance levels and mechanisms to penoxsulam and cyhalofop-butyl in barnyardgrass (Echinochloa crus-galli) from Ningxia Province, China
复制标题
宁夏稗草对五氟磺草胺和氰氟草酯的抗性水平及机制研究
DOI：
10.1017/wsc.2021.37
复制
发表时间：
2021-05
期刊：
影响因子：
2.5
通讯作者：
Li Yongfeng
中科院分区：
文献类型：
--
作者：
Yang Qian;Yang Xia;Zhang Zichang;Wang Jieping;Fu Weiguo;Li Yongfeng
稗草[Echinochloa crus-galli（L.）P. Beauv.]是一种有害的禾本科杂草，在稻田中滋生，造成巨大的农作物产量损失。本研究收集了12株E.本研究以乙酰乳酸合成酶（ALS）抑制剂五氟磺草胺（penoxsulam）和乙酰辅酶A羧化酶（ACCase）抑制剂氰氟草酯（cyhalofop-butyl）为抗药性指标，测定了宁夏稻田稻瘟病菌种群对乙酰乳酸合成酶（ALS）抑制剂五氟磺草胺和乙酰辅酶A羧化酶（ACCase）抑制剂氰氟草酯的抗性水平。结果表明，8个种群对五氟磺草胺表现出抗性，4个种群对氰氟草酯产生抗性。resistance.to此外，所有4个氰氟草酯抗性群体（NX 3、NX 4、NX 6和NX 7）均对五氟磺草胺和氰氟草酯表现出多重抗性。替代除草剂双草醚、精恶唑禾草灵和精恶唑禾草灵不能有效地防治多抗性植物。为了表征抗性的分子机制，我们扩增并测序了抗性和易感群体中的靶位点编码基因。检测了3个ALS基因和6个ACCase基因的部分序列。在两个高水平（65.84和59.30倍）的五氟磺仑耐药群体，NX 2和NX 10，分别在EcALS 1和EcALS 3中检测到Trp-574-Leu突变。此外，ACC酶基因的一个拷贝（EcACC 4）编码一个截短的异常蛋白，这是由于E.鸡群在MHR群体的ALS和ACCase基因中没有known.to我们的研究揭示了MHR E的广泛传播。宁夏省的稗草种群对几种ALS和ACCase抑制剂表现出抗性。非靶位点机制可能参与E.在四个MHR群体中，至少存在对除草剂的抗性。
Barnyardgrass [Echinochloa crus-galli (L.) P. Beauv.] is a noxious grass weed that infests rice.fields and causes huge crop yield losses. In this study, we collected 12 E. crus-galli populations.from rice fields of Ningxia Province in China and investigated the resistance levels to the.acetolactate synthase (ALS) inhibitor penoxsulam and the acetyl-CoA carboxylase (ACCase).inhibitor cyhalofop-butyl. The results showed that eight populations exhibited resistance.to penoxsulam and four populations evolved resistance to cyhalofop-butyl. Moreover, all four.cyhalofop-butyl–resistant populations (NX3, NX4, NX6, and NX7) displayed multiple.herbicide resistance to both penoxsulam and cyhalofop-butyl. The alternative herbicides.bispyribac-sodium, metamifop, and fenoxaprop-p-ethyl cannot effectively control the multiple.herbicide–resistant (MHR) plants. To characterize the molecular mechanisms of resistance,.we amplified and sequenced the target site–encoding genes in resistant and susceptible.populations. Partial sequences of three ALS genes and six ACCase genes were examined..A Trp-574-Leu mutation was detected in EcALS1 and EcALS3 in two high-level (65.84- and.59.30-fold) penoxsulam-resistant populations, NX2 and NX10, respectively. In addition, one.copy (EcACC4) of ACCase genes encodes a truncated aberrant protein due to a frameshift.mutation in E. crus-galli populations. None of the amino acid substitutions that are known.to confer herbicide resistance were detected in ALS and ACCase genes of MHR populations..Our study reveals the wide spread of MHR E. crus-galli populations in Ningxia Province that.exhibit resistance to several ALS and ACCase inhibitors. Non–target site based mechanisms.are likely to be involved in E. crus-galli resistance to the herbicides, at least in four MHR.populations.
登录
查看更多内容
使用下一代焦磷酸测序技术对稗草（稗草）转录组进行从头组装和表征
影响因子：
3.7
作者：
Yang X;Yu XY;Li YF
通讯作者：
Li YF
影响因子：
5.6
作者：
Iwakami S;Shimono Y;Manabe Y;Endo M;Shibaike H;Uchino A;Tominaga T
通讯作者：
Tominaga T
影响因子：
3.5
作者：
Gao, Yuan;Pan, Xukun;Dong, Liyao
通讯作者：
Dong, Liyao
影响因子：
2.5
作者：
Deng, Wei;Di, Yingjie;Yuan, Shuzhong
通讯作者：
Yuan, Shuzhong
影响因子：
4.6
作者：
Xia X;Tang W;He S;Kang J;Ma H;Li J
通讯作者：
Li J