Liquid shake culture overcomes solid plate culture in inducing conidial production of Colletotrichum isolates
Liquid shake culture overcomes solid plate culture in inducing conidial production of Colletotrichum isolates
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液体振荡培养在诱导炭疽菌分离株的分生孢子产生方面优于固体平板培养
DOI：
10.1007/s13313-017-0490-3
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发表时间：
2017-05
影响因子：
1.4
通讯作者：
Sun Guangyu
中科院分区：
文献类型：
--
作者：
Wang W.;Liang X.;Zhang Rong;Gleason M. L.;Sun Guangyu
炭疽菌属的真菌对许多重要经济水果、蔬菜和观赏作物造成破坏性炭疽病。不幸的是，许多分离株无法在人工培养基上形成孢子的事实限制了需要分生孢子的研究。我们发现，液体培养有效地挽救了炭疽菌分离株的分生孢子生产，而在固体平板培养基上却无法做到这一点，四天后产量平均为每毫升 107 个。我们确定最有效的培养方案是马铃薯葡萄糖肉汤 (PDB) 在 25 °C 下摇动培养 (140 rpm)。此外，必须使用足够的培养基来覆盖菌丝球。该方法适用于来自一系列宿主的不同炭疽菌物种。总之，我们在这里报告，液体摇瓶培养提供了一种诱导“非孢子化”炭疽菌分离株产生分生孢子的替代方法，这是实验室许多研究活动的关键步骤。
Species of fungi in the genus Colletotrichum cause damaging anthracnose diseases on many economically important fruit, vegetable, and ornamental crops. Unfortunately, the fact that many isolates fail to sporulate on artificial media limits research requiring conidia. We found that liquid culture efficiently rescued conidial production of Colletotrichum isolates being impotent to do so on solid plate media, with the yield averaging 107 per mL after four days. We determined that the most efficient culture protocol was potato dextrose broth (PDB) shake culture (140 rpm) at 25 °C. In addition, it was essential that enough medium was used to cover the mycelial balls. The method was applicable to different Colletotrichum species from a range of hosts. In sum, we report here that liquid shake culture offers an alternative in inducing conidia production of ‘non-sporulating’ Colletotrichum isolates, a critical step for many research activities in the lab.
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DOI：
10.4324/9781315172538-4
发表时间：
2018-10
期刊：
The Groupwork Manual
影响因子：
--
作者：
Andy Hickson
通讯作者：
Andy Hickson
影响因子：
16.5
作者：
Cannon PF;Damm U;Johnston PR;Weir BS
通讯作者：
Weir BS
影响因子：
1.5
作者：
T. Furukawa;K. Kishi
通讯作者：
T. Furukawa;K. Kishi
影响因子：
2.7
作者：
W. Vishniac
通讯作者：
W. Vishniac
影响因子：
--
作者：
J. Soltani;M. Haghighi;S. Nazeri
通讯作者：
J. Soltani;M. Haghighi;S. Nazeri