Differentially Expressed mRNAs and Their Long Noncoding RNA Regulatory Network with Helicobacter pylori-Associated Diseases including Atrophic Gastritis and Gastric Cancer.
Differentially Expressed mRNAs and Their Long Noncoding RNA Regulatory Network with Helicobacter pylori-Associated Diseases including Atrophic Gastritis and Gastric Cancer.
复制标题
DOI：
10.1155/2020/3012193
复制
发表时间：
2020
影响因子：
--
通讯作者：
Gong Y
中科院分区：
文献类型：
--
作者：
Liu S;Yin H;Zheng S;Chu A;Li Y;Xing C;Yuan Y;Gong Y
幽门螺杆菌（Hp）感染是胃癌（GC）的最大危险因素。然而，hp相关GC的机制仍有待探索。从GEO数据库下载基因表达谱（GSE111762）数据。用GEO2R法鉴定正常样品（NO）与hp -萎缩性胃炎（GA）或Hp-GA与Hp-GC之间的差异表达基因（DEGs）。使用DAVID数据库进行基因本体和途径富集分析。利用Cytoscape构建lncRNA-TF-mRNA和ceRNA调控网络。交叉网络是由上述两个网络的分子重叠得到的。采用GSE27411和GSE116312数据集进行验证。NO和Hp-GA之间的deg与向内整流钾通道的活性、消化等有关。Hp-GA和Hp-GC之间的deg与消化、细胞增殖的正向调节等相关。根据lncRNA-TF-mRNA网络，Hp-GA中包含63个lncrna、12个tf和209个mrna， Hp-GC网络中包含16个lncrna、11个tf和92个mrna。在ceRNA网络中，Hp-GA中包含120个mrna、18个mirna和27个lncRNA， Hp-GC网络中包含72个mrna、8个mirna和1个lncRNA。在交叉网络中，我们发现免疫调节和分化调节在NO-GA过程中起重要作用。神经内分泌调节主要与GA-GC过程有关。最后，我们验证了CDX2在NO to Hp-GA的病理过程中发挥了重要作用。Hp-GA与Hp-GC比较，DEGs （FPR1、TFF2、GAST、SST、FUT9和SHH）、TF和GATA5具有重要意义。我们确定了这些deg，它们的Hp相关疾病的lncRNA调控网络可能为Hp感染和GC之间的机制提供见解。此外，对这些分子的深入研究可能有助于探索胃疾病的多步骤过程。
Helicobacter pylori (Hp) infection is the strongest risk factor for gastric cancer (GC). However, the mechanisms of Hp-associated GC remain to be explored. The gene expression profiling (GSE111762) data were downloaded from the GEO database. Differentially expressed genes (DEGs) between normal samples (NO) and Hp-atrophic gastritis (GA) or Hp-GA and Hp-GC were identified by GEO2R. Gene Ontology and pathway enrichment analysis were performed using the DAVID database. lncRNA-TF-mRNA and ceRNA regulation networks were constructed using Cytoscape. The cross-networks were obtained by overlapping molecules of the above two networks. GSE27411 and GSE116312 datasets were employed for validation. DEGs between NO and Hp-GA are linked to the activity of inward rectifying potassium channels, digestion, etc. DEGs between Hp-GA and Hp-GC were associated with digestion, positive regulation of cell proliferation, etc. According to the lncRNA-TF-mRNA network, 63 lncRNAs, 12 TFs, and 209 mRNAs were involved in Hp-GA while 16 lncRNAs, 11 TFs, and 92 mRNAs were contained in the Hp-GC network. In terms of the ceRNA network, 120 mRNAs, 18 miRNAs, and 27 lncRNAs were shown in Hp-GA while 72 mRNAs, 8 miRNAs, and 1 lncRNA were included in the Hp-GC network. In the cross-network, we found that immune regulation and differentiation regulation were important in the process of NO-GA. Neuroendocrine regulation was mainly related to the process of GA-GC. In the end, we verified that CDX2 plays an important role in the pathological process of NO to Hp-GA. Comparing Hp-GA with Hp-GC, DEGs (FPR1, TFF2, GAST, SST, FUT9, and SHH), TF, and GATA5 were of great significance. We identified the DEGs, and their lncRNA regulatory network of Hp-associated diseases might provide insights into the mechanism between Hp infection and GC. Furthermore, in-depth studies of the molecules might be useful to explore the multistep process of gastric diseases.
登录
查看更多内容
影响因子：
37.3
作者：
Gibb EA;Brown CJ;Lam WL
通讯作者：
Lam WL
影响因子：
3.7
作者：
Herrera-Martinez, Aura D.;Gahete, Manuel D.;Castano, Justo P.
通讯作者：
Castano, Justo P.
影响因子：
6.4
作者：
Holleczek, Bernd;Schoettker, Ben;Brenner, Hermann
通讯作者：
Brenner, Hermann
影响因子：
82.9
作者：
Kononen, J;Bubendorf, L;Kallioniemi, OP
通讯作者：
Kallioniemi, OP
影响因子：
6.4
作者：
Camilo, Vania;Garrido, Monica;Almeida, Raquel
通讯作者：
Almeida, Raquel