Analysis of rice ER-resident J-proteins reveals diversity and functional differentiation of the ER-resident Hsp70 system in plants.
Analysis of rice ER-resident J-proteins reveals diversity and functional differentiation of the ER-resident Hsp70 system in plants.
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DOI：
10.1093/jxb/ert312
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发表时间：
2013-12
影响因子：
6.9
通讯作者：
Takaiwa F
中科院分区：
文献类型：
--
作者：
Ohta M;Wakasa Y;Takahashi H;Hayashi S;Kudo K;Takaiwa F
热休克蛋白70 （Hsp70）伴侣系统参与蛋白质折叠和未折叠蛋白的质量控制。为了研究共伴侣蛋白在水稻内质网Hsp70伴侣蛋白系统中的作用，研究了6种内质网j蛋白（OsP58A、OsP58B、OsERdj2、OsERdj3A、OsERdj3B和OsERdj7）在水稻中的功能。在内质网胁迫下，根中OsP58B、OsERdj3A和OsERdj3B的表达明显上调。这种反应是通过ATF6同源物如OsbZIP39和OsbZIP60介导的，而不是通过IRE1/OsbZIP50途径介导的。共免疫沉淀实验表明，OsP58A、OsP58B和OsERdj3B优先与主要OsBiP OsBiP1相互作用，而OsERdj3A优先与OsBiP5相互作用，表明OsBiPs与j蛋白之间存在不同的亲和力。在胚乳组织中，OsP58A、OsP58B和OsERdj2主要定位于内质网，而OsERdj2则定位于内质网衍生蛋白体（PB-Is）的外表面。此外，OsERdj3A在野生型种子中不表达，但在积累人白细胞介素-7 （hIL-7）的转基因种子中表达上调。由于erdj3a -绿色荧光蛋白（GFP）也在内质网胁迫条件下的伤组织细胞液泡中检测到，因此OsERdj3A是一个真正的液泡定位蛋白。OsP58A、OsP58B和OsERdj3A在表达不同重组蛋白的转基因植物中积累差异较大。这些结果揭示了水稻ER-resident Hsp70系统的功能多样性。
The heat shock protein 70 (Hsp70) chaperone system participates in protein folding and quality control of unfolded proteins. To examine the roles of co-chaperones in the rice Hsp70 chaperone system in the endoplasmic reticulum (ER), the functions of six ER-resident J-proteins (OsP58A, OsP58B, OsERdj2, OsERdj3A, OsERdj3B, and OsERdj7) in rice were investigated. The expression of OsP58B, OsERdj3A, and OsERdj3B was predominantly up-regulated in roots subjected to ER stress. This response was mediated by signalling through ATF6 orthologues such as OsbZIP39 and OsbZIP60, but not through the IRE1/OsbZIP50 pathway. A co-immunoprecipitation assay demonstrated that OsP58A, OsP58B, and OsERdj3B preferentially interact with the major OsBiP, OsBiP1, while OsERdj3A interacts preferentially with OsBiP5, suggesting that there are different affinities between OsBiPs and J-proteins. In the endosperm tissue, OsP58A, OsP58B, and OsERdj2 were mainly localized in the ER, whereas OsERdj2 was localized around the outer surfaces of ER-derived protein bodies (PB-Is). Furthermore, OsERdj3A was not expressed in wild-type seeds but was up-regulated in transgenic seeds accumulating human interleukin-7 (hIL-7). Since ERdj3A–green fluorescent protein (GFP) was also detected in vacuoles of callus cells under ER stress conditions, OsERdj3A is a bona fide vacuole-localized protein. OsP58A, OsP58B and OsERdj3A were differentially accumulated in transgenic plants expressing various recombinant proteins. These results reveal the functional diversity of the rice ER-resident Hsp70 system.
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通讯作者：
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