Comparative transcriptional and functional profiling of clear cell and papillary renal cell carcinoma.
Comparative transcriptional and functional profiling of clear cell and papillary renal cell carcinoma.
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透明细胞癌和乳头状肾细胞癌的转录和功能比较。
DOI：
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发表时间：
2006
影响因子：
5.4
通讯作者：
F. von Eggeling
中科院分区：
文献类型：
--
作者：
J. Diegmann;S. Tomiuk;J. Sanjmyatav;K. Junker;W. Hindermann;F. von Eggeling
已知肾细胞癌（RCC）有效地阻止免疫识别。然而，人们对这种现象背后的机制知之甚少。因此，与RCC相关的免疫原性分子的鉴定和相应的信号通路的阐明是至关重要的有效治疗的发展。我们用cDNA微阵列（1070个cDNA探针）对17个RCC（11个透明细胞和6个乳头状细胞）和相应的正常组织进行了转录和功能分析。样品基于其表达谱聚类。我们发现，与正常组织相比，两种肿瘤类型共调节了45个基因。一组13个差异表达的基因被确定之间的检查肿瘤亚型。对两个基因集进行功能分析，结果显示两种肿瘤亚型中调节的细胞表面基因显著富集。在这些基因中，我们发现了5个上调的表面标记基因（TNFRSF10B、CD70、TNFR1、PDGFRB和BAFF），它们通过调节淋巴细胞参与免疫应答，也可以诱导细胞凋亡。它们在两种肿瘤亚型中的过表达表明可能参与RCC的免疫逃逸策略。转录和功能分析的结合揭示了新的治疗策略的潜在靶分子，必须进行更详细的研究。
Renal cell carcinoma (RCC) is known to effectively prevent immune recognition. However, little is known about the mechanisms that underlie this phenomenon. Thus, the identification of immunogenic molecules associated with RCC and the elucidation of the corresponding signaling pathways are crucial to the development of effective treatments. We performed transcriptional and functional profiling with cDNA microarrays (1070 cDNA probes) on a total of 17 RCCs, 11 clear cell and 6 papillary, and on corresponding normal tissue. Samples were clustered based on their expression profiles. We found a total of 45 genes to be regulated equally by both tumor types compared to the normal tissue. A set of 13 differentially expressed genes was identified between the examined tumor subtypes. Functional analysis was performed for both gene sets and showed a significant enrichment of cell surface genes regulated in both tumor subtypes. Within these we found five surface marker genes to be upregulated (TNFRSF10B, CD70, TNFR1, PDGFRB, and BAFF) which are involved in immune responses via the regulation of lymphocytes and can also induce apoptosis. Their overexpression in both tumor subtypes suggests a possible involvement in the immune escape strategies of RCC. The combination of transcriptional and functional profiling revealed potential target molecules for novel therapy strategies that must be studied in more detail.
影响因子：
12.3
作者：
Martins FC;Santiago Id;Trinh A;Xian J;Guo A;Sayal K;Jimenez-Linan M;Deen S;Driver K;Mack M;Aslop J;Pharoah PD;Markowetz F;Brenton JD
通讯作者：
Brenton JD
影响因子：
6
作者：
Higgins, JPT;Shinghal, R;Brooks, JD
通讯作者：
Brooks, JD
DOI：
10.1073/pnas.95.25.14863
发表时间：
1998-12-08
影响因子：
11.1
作者：
Eisen, MB;Spellman, PT;Botstein, D
通讯作者：
Botstein, D